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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Sequence optimization and multiple gene-targeting improve the inhibitory efficacy of exogenous double-stranded RNA against pepper mottle virus in Nicotiana benthamiana

Fig. 5

Co-treatment of two dsRNAs successfully delayed PepMoV infection in N. benthamiana. a PepMoV infection status of dsRNA mixture-treated plants at 5/8/10 dpi. dsRNA mixture for infiltration was prepared by mixing 15 µg of each short dsRNA compartment (selected from the results shown in Fig. 2. and Fig. 3). GFP signals (bright-green colors in images of 5-dpi and 8-dpi plants) represent infectious PepMoV clones inside the plants. b GFP transcript levels measured by quantitative PCR in the local leaves of 8-dpi co-treated plants. R.luc indicates the plants treated with dsRNA containing partial sequences (500-bp-long) of Renilla luciferase. Data are presented as mean (± SEM; n = 3). Significance is determined by Student’s t-test

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