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Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Therapeutic effects of TMF and catechol in pulmonary fibrosis: in vitro and in vivo analysis

Fig. 4

TMF and CAT result in a decrease of BLM-induced fibrosis and NOX2 expression. A, B BLM (20 μg/ml) significantly increased the NOX2 expression under both 24 and 48 h treatments. In the 24 h treatment, the change of non-canonical signaling pathways' factors were observed. TMF and CAT alleviated the stimulated PI3K/AKT, ERK and p38 pathways. In the 48 h treatment, the antifibrotic and anti-EMT effect of TMF and CAT were confirmed. The graph shows the relatively quantified value of western blot results if that of the control group against actin was 1. C In the Sirius Red staining assay, BLM for 5 d increased collagen production in A549 cells, as can be seen by the increase of red intensity. TMF and CAT reduced that increase. The graph shows the relative red intensity in the 100 × microscopic images. D In the transwell migration assay, we could observe TMF and CAT inhibiting EMT of A549 cells induced by BLM (20 μg/ml, 72 h). We counted the number of invasive cells across the permeable membrane from the upper layer of cell culture during the treatment

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