Fig. 5
From: Therapeutic effects of TMF and catechol in pulmonary fibrosis: in vitro and in vivo analysis
TMF and CAT show effects against fibrosis and inflammation in BLM-challenged mice lungs. A Representative images of pulmonary tissues stained with hematoxylin and eosin (H&E) or Masson’s trichrome staining. Each specimen was photographed under 50 × microscopy. The graph shows the Ashcroft score which was assigned based on those two types of staining images. B Western blot results of homogenized lung tissue show antifibrotic effects of TMF and CAT. The bands here include two samples for each treatment group and the graph reflects two of each, showing their average. C The concentration of TGF-β1 (pg/ml) in mice serum of each group was investigated by sandwich ELISA and the graph shows the average calculated value. D Analysis of total cell numbers and types (NEU, LYM and macrophage) in BALF. BALF analysis was performed in mice after three weeks of treatment of each. E qRT-PCR results using lung tissue show the normalized fold mRNA expression of IL-1β, IL-13 and TNF-α relative to actin