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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Cherry fruit anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside protect against blue light-induced cytotoxicity in HaCaT cells

Fig. 2

Effects of C3OG and C3OR on ROS production in HaCaT cells exposed to blue light. HaCaT cells cultured in 96-well plates were exposed to blue light (20,000 lx) for 1 h and then stained with H2DCFDA (A, B). HaCaT cells were pre-treated with the indicated concentrations of C3OG or C3OR for 24 h or with NAC (10 mM) for 1 h prior to exposure to blue light (A, B). The generation of ROS was visualized using a fluorescence microscope, Scale bar, 100 μm (A), or measured using a fluorescence plate reader (B). The bar graph presents the fold changes in ROS production in cells exposed to blue light relative to the unexposed control cells (n = 3, ±SD). ** p < 0.01 and *** p < 0.001 vs. blue light (20,000 lx)

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