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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Cherry fruit anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside protect against blue light-induced cytotoxicity in HaCaT cells

Fig. 3

Effects of C3OG and C3OR on the activation of FAK and MAPK induced by blue light. HaCaT cells in six-well plates were exposed to blue light (20,000 lx) for 30 min. Cells were pre-treated with or without NAC (10 mM) and FAK-i (1 µM) for 1 h or different concentrations (10, 50, 100, or 200 µM) of C3OG or C3OR for 24 h prior to blue light exposure (A). Immunoblots show pY397 FAK, p-IKKα, p-JNK, p-ERK, and p-p38, the corresponding total proteins, and GAPDH used as a loading control. B–D Calculated fold changes in pY397 FAK, p-IKKα, p-JNK, p-ERK, and p-p38 (n = 3, ±SD). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. blue light (20,000 lx)

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