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Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Cherry fruit anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside protect against blue light-induced cytotoxicity in HaCaT cells

Fig. 5

Effects of C3OG and C3OR on blue light-induced FAK cleavage and caspase-3 activation. HaCaT cells in six-well plates were exposed to blue light for 1 h and then treated with or without NAC (10 mM) or z-DEVD-fmk (200 µM) for 24 h. Cells were pre-treated with 3OG (200 µM) or C3OR (200 µM) for 24 h prior to blue light exposure. (A) Immunoblots of total-FAK, PARP, procaspase-3, active caspase-3 (cleaved), and the GAPDH loading control are shown. (BD) Calculated fold changes of cleaved-FAK, -PARP, and -caspase-3 calculated (n = 3, ±SD). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. blue light (20,000 lx)

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