Fig. 2

Suppression of Phytophthora capsici infection and PcNLP2 expression via PcNLP2-targeting dsRNA. a The left figure shows the scheme of dsRNA treatment and P. capsici infection, and the right figure shows the phenotype result of P. capsici infection after treatment with mock and dsRNA_PcNLP2_5′, respectively, in 3 week-old Nicotiana benthamiana leaves using a fluorescence in vivo imaging system FOBI. Scale bar = 1 cm. b Quantification of P. capsici infection lesion size using ImageJ. c Quantification of PcNLP2 transcript level using quantitative real-time PCR (qRT-PCR). Mock: Treated with dsRNA targeting Renilla luciferase; dsRNA_PcNLP2_5′: Treated with dsRNA_PcNLP2_5′. Data represent mean ± standard error of mean (SEM; N = 3). Significance is determined by Student’s t-test, *P < 0.05 and **P < 0.01