Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

Applied Biological Chemistry

Table 1 Salient features of various Cas proteins used in diagnostics

Cas Protein	Class	Target	PAM	Collateral Activity	Refs.
Cas9	Class 2	dsDNA	NGG	No	[85]
Cas12a	Class 2	Both (ss/dsDNA)	TTTN	Yes (ssDNA)	[5, 18, 86]
Cas12b	Class 2	Both (ss/dsDNA)	TTN	Yes (ssDNA)	[6]
Cas13a	Class 2	ssRNA	–	Yes (ssRNA)	[5]
Cas13d	Class 2	ssRNA	–	Yes (ssRNA)	[87]
Cas14a	Class 2	ssDNA	–	Yes (ssDNA)	[3]