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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Knockdown of circ_0006872 alleviates CSE-induced human bronchial epithelial cells injury in chronic obstructive pulmonary disease

Fig. 5

CDKN1B is a target of miR-485-3p, and circ_0006872/miR-485-3p/CDKN1B constitutes a feedback loop in 16HBECs. A The conserved target site of miR-485-3p on CDKN1B predicted by starbase software was listed. B, C The interaction between miR-485-3p and CDKN1B was confirmed using dual-luciferase reporter assay and RNA pull-down assay. D Levels of CDKN1B mRNA in non-smokers (n = 35), smokers (n = 35), and smokers with COPD (n = 35). E The correlation between miR-485-3p and CDKN1B mRNA expression level in smokers with COPD (n = 35) was assessed by Pearson’s correlation coefficient assay (r=-0.8606, p < 0.001). F CDKN1B protein level in non-smokers (n = 35), smokers (n = 35), and smokers with COPD (n = 35). G CDKN1B expression in 16HBECs exposed to different concentrations of CSE. H The impacts of circ_0006872/miR-485-3p axis on CDKN1B expression in 16HBECs under CSE treatment. **P < 0.01, ***P < 0.001, ****P < 0.0001

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