Development of analytical method for veterinary antibiotics and monitoring of residuals in agricultural environment

Applied Biological Chemistry

Table 1 LC–MS/MS parameters with MRM transitions for analysis of 12 antibiotics

Class	Compound (abbreviation)	Precursor ion (m/z)	Product ion (m/z)	DP¹⁾ (V)	EP²⁾ (V)	CE³⁾ (V)	CXP⁴⁾ (V)
	Simeton⁵⁾		124.1⁶⁾			27	6
		198.1	128.1	71	10	27	22
			100.1			39	18
Penicillins	Amoxicillin (AMO)		349.1			11	8
		365.9	114.0	6	10	23	8
			134.0			37	12
	Ampicillin (AMP)		106.1			41	14
		349.9	192.0	6	10	21	24
			113.9			41	8
	Penicillin G (PNG)		217.0			19	12
		334.9	202.0	136	10	31	24
			91.0			67	16
Tetracyclines	Chlortetracycline (CTC)		444.0			31	24
		479.0	462.1	66	10	23	12
			260.0			73	18
	Oxytetracycline (OTC)		426.1			27	18
		461.1	443.2	36	10	19	22
			201.1			49	12
	Tetracycline (TC)		410.1			29	20
		445.1	427.1	26	10	19	28
			154.2			37	14
Macrolides	Spiramycin (SPM)		174.2			27	8
		843.5	540.3	101	10	19	14
			101.0			23	10
	Tylosin (TYL)		772.4			45	4
		916.5	174.2	61	10	45	22
			83.1			129	2
Sulfonamides	Sulfadiazine (SDZ)		156.0			21	8
		251.0	92.0	1	10	33	12
			65.0			61	6
	Sulfamethoxazole (SMX)		186.0			23	10
		279.0	124.0	1	10	29	12
			65.0			67	8
	Sulfamethazine (SMZ)		156.0			21	14
		254.0	92.0	1	10	33	10
			65.0			61	6
	Sulfathiazole (STZ)		155.9			21	18
		255.9	92.0	21	10	33	12
			65.0			63	6

¹⁾ DP decluttering potential, ²⁾ EP Entrance potential, ³⁾ CE Collision energy, ⁴⁾ CXP Collision Cell Exit Potential, ⁵⁾ simeton internal standard, ⁶⁾ Bold product ion was used for quantification