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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Antimicrobial photodynamic therapy with Ligularia fischeri against methicillin-resistant Staphylococcus aureus infection in Caenorhabditis elegans model

Fig. 5

Effect of APDT-LFE on the growth of C. elegans in the prefeeding model. S. aureus KCTC3881 or MRSA was pretreated with APDT using LFE (20 µg/mL; 30 min incubation) and red light 120 W/m2 for 15 min. The treatment with ampicillin (100 µg/mL) served as the positive control. Synchronized eggs were fed with E. coli OP50 as vehicle control, or treated MRSA or S. aureus with APDT or ampicillin. The body length A, C and the microscopic images of the worms B, D were evaluated four days after the egg state. White scale bar = 1 mm. The data are representative of three independent experiments. Analysis of variance test (n = 20). ns indicates no statistical significance relative to the control group; significant differences are expressed with *** for p < 0.001 relative to the vehicle control group; ## for p < 0.01, ### for p < 0.001 relative to the pathogen single treatment group

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