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Applied Biological Chemistry

Table 4 Single-turnover kinetic parameters for the reaction of MauG toward the novel and original substrate

From: Identification of 7-hydroxyindole as an alternative substrate of MauG by in silico and in vitro analysis

 

Single-turnover kinetic parameters

References

k3 (s−1)

Kd (μM)

k4 (s−1)

7-hydroxyindole

1.23 ± 0.05

28.16 ± 7.60

−0.32 ± 0.07

In this study

Pre-MADH

0.80 ± 0.10

≤ 1.50

Not mentioned

[17]

5-hydroxyindole

2.66 ± 0.33

3986 ± 1143

Not mentioned

[30]

6-hydroxyindole

4.87 ± 0.20

2470 ± 280

Not mentioned

[30]

  1. The values are obtained from the plot (Fig. 4B) using Eq. (1)
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