Fig. 4

Subcellular localization of BBX proteins. A. GFP fusion proteins were constitutively expressed in Arabidopsis mesophyll protoplasts. B. Schematic representation of the GST-fused BBX protein expression cassettes (left panel). GFP signals in tobacco leaf epidermal cells. Scale bars = 50 μm. C. In planta interactions between CO and (1) BBX30 and (2) BBX31. The N-YFP fragment was fused with CO while the C-YFP fragment was fused with BBX30 and BBX3. The C-YFP-CO/N-YFP-BBX30 and C-YFP-CO/N-YFP-BBX31 combinations were co-expressed in tobacco leaves. The YFP signals indicate that CO interacts with BBX30 and BBX31 in the nucleus. Zoom images show nuclear speckle formation. Scale bars = 50 μm