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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Transcriptome profiling uncovers the involvement of CmXyn1, a glycosyl hydrolase 11, in Cochliobolus miyabeanus pathogenicity

Fig. 3

Characterization of a putative effector of C. miyabeanus, glycoside hydrolase11 (CmXyn1). A Schematic presentation of the vector constructs used, including those containing the signal sequence (pFL-CmXyn1) and those without it (pNS-CmXyn1). B A positive control (HopZ), an empty vector control (pGWB517, EV), constructs with FL/NS-CmXyn1 used for agro-infiltration. C DAB-staining of N. benthamiana leaves showed an accumulation of ROS. D Validation of anti-α-MYC specific GH11 expression levels in N. benthamiana leaves infiltrated with FL/NS-CmXyn1 using Western blotting, with a loading control. E Validation of pathogenesis-related genes (PR1 and PR5) in N. benthamiana leaves transiently infected with FL/NS-CmXyn1 using qRT-PCR (**p < 0.01, ***p < 0.001)

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