Skip to main content

Anti-inflammatory and anticancer properties of dichloromethane and butanol fractions from the stem bark of Broussonetia papyrifera

Abstract

Broussonetia papyrifera radix, fruits, leaves, and stems exhibit antioxidant, antinociceptive, antityrosinase, anti-inflammatory, and antiplatelet activities. However, studies of the anticancer and anti-inflammatory activities of B. papyrifera stem bark are limited. Yet it is important to evaluate these activities to isolate active compounds for potential medicines and food additive. The dichloromethane fraction of B. papyrifera stem bark was found to induce apoptosis-related DNA fragmentation, increase sub-G1 accumulation, increase morphological changes, and inhibit the proliferation of human colon cancer HT-29 cells. Moreover, it significantly increased p53, caspase-3, and Bax expression in HT-29 cells. In addition, the n-butanol fraction inhibited nitric oxide production in RAW 264.7 macrophages by suppressing iNOS expression. These results suggest that B. papyrifera is a potential natural resource for treating cancer and inflammatory diseases.

References

  • Aktan F (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci 75, 639–653.

    CAS  Article  Google Scholar 

  • Ayed-Boussema I, Bouaziz C, Rjiba K, Valenti K, Laporte F, Bacha H, and Hassen W (2008) The mycotoxin Zearalenone induces apoptosis in human hepatocytes (HepG2) via p53-dependent mitochondrial signaling pathway. Toxicol In Vitro 22, 1671–1680.

    CAS  Article  Google Scholar 

  • Chen RM, Hu LH, An TY, Li J, and Shen Q (2002) Natural FTP1B Inhibitors from Broussonet papyrifera. Bioorg Med Chem Lett 12, 3387–3390.

    CAS  Article  Google Scholar 

  • Chiu SM, Xue LY, Usuda J, Azizuddin K, and Oleinick NL (2003) Bax is essential for mitochondrion-mediated apoptosis but not for cell death caused by photodynamic therapy. Brit J Cancer 89, 1590–1597.

    CAS  Article  Google Scholar 

  • Earnshaw WC, Martins LM, and Kaufmann SH (1999) Mammalian caspases: Structure, activation, substrates, and functions during apoptosis. Annu Rev Biochem 68, 383–424.

    CAS  Article  Google Scholar 

  • Hanson KD, Shichiri M, Follansbee M, and Sedivy J M. (1994) Effects of c-myc expression on cell cycle progression. Mol Cell Biol 9, 5748–5755.

    Google Scholar 

  • Ko HH, Chang WL, and Lu TM (2008) Antityrosinase and antioxidant effects of kaurane diterpenes from leaves of Broussonetia papyrifera. J Nat Prot 71, 1930–1933.

    CAS  Article  Google Scholar 

  • Khan MR and Mlungwana SM (1999) c-sitosterol, a cytotoxic sterol from markhamia zanzibarica and kigelia africana. Fitoterapia 70, 96–97.

    CAS  Article  Google Scholar 

  • Lee D, Bhat KL, Fong HS, Farnsworth NR, Pezzuto JM, and Kinghorn AD (2001) Aromatase inhibitors from Broussonetia papyrifera. J Nat Prot 64, 1286–1293.

    CAS  Article  Google Scholar 

  • Lin CN, Lu CM, Lin HC, Fang SC, Shieh BJ, Hsu MF, Wang JP, Ko FN, and Teng CM (1996) Novel antiplatelet constituents from Formosan Moraceous plants. J Nat Prot 59, 834–838.

    CAS  Article  Google Scholar 

  • Lin LW, Chen HY, and Wu CR (2008) Comparison with various parts of broussonetia papyrifera as to the antinociceptive and anti-inflammatory activlities in rodents. Biosci Biotechnol Biochem 72, 2377–2384.

    CAS  Article  Google Scholar 

  • MacMicking J, Xie QW, and Nathan C (1997) Nitric oxide and macrophage function. Annu Rev Immunol 15, 323–350.

    CAS  Article  Google Scholar 

  • Mei RQ, Wang YH, Du GH, Liu GM, Zhang L, and Cheng YX (2009) Antioxidant lignans from the fruits of Broussonetia papyrifera. J Nat Prot 72, 621–625.

    CAS  Article  Google Scholar 

  • Nagai H, Kumamoto H, Fukuda M, and Takahashi T (2003) Inducible nitric oxide synthase and apoptosis-related factors in the synovial tissues of temporomandibular joints with internal derangement and osteoarthritis. J Oral Maxil Surg 61, 801–807.

    Article  Google Scholar 

  • Panchal RG (1998) Novel therapeutic strategies to selectively kill cancer cells. Biochem Pharmacol 55, 247–252.

    CAS  Article  Google Scholar 

  • Petros A, Bennett D, and Vallance P (1991) Effect of nitric oxide synthase inhibitors on hypotension in patients with septic shock. Lancet 338, 1557–1558.

    CAS  Article  Google Scholar 

  • Ramos S (2007) Effects of dietary flavonoids on apoptotic pathways related to cancer chemoprevention. J Nutr Biochem 18, 427–442.

    CAS  Article  Google Scholar 

  • Shah S, Gapor A, and Sylvester PW (2003) Role of caspase-8 activation in mediating vitamin E-induced apoptosis in murine mammary cancer cells. Nutr Cancer 45, 236–246.

    CAS  Article  Google Scholar 

  • Wyllie AH (1997) Apoptosis: An overview. Brit Med Bull 53, 451–465.

    CAS  Google Scholar 

  • Xie QW, Cho HJ, Calaycay J, Mumford RA, Swiderek KM, Lee TD, Ding A, Troso T, and Nathan C (1992) Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256, 225–228.

    CAS  Article  Google Scholar 

  • Xu DH, Yang Y, Zhang XG, Zhang XH, Zh YN, Zheng ZP, Zhou Y, Tang W, Yang YF, Hu LH, and Zuo JP (2008) Anti-inflammatory effects of Z23 on LPS-induced inflammatory responses in AW264.7 macrophages. J. Ethnopharmacol 120, 447–451.

    Article  Google Scholar 

  • Yin Y, Heo SI, Roh KS, and Wang MH (2009) Biological activities of fractions from methanolic extract of Picrasma quassioides. J Plant Biol 52, 325–331.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Myeong-Hyeon Wang.

Additional information

These authors are equally contributed to this work

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wang, L., Son, H.J., Xu, ML. et al. Anti-inflammatory and anticancer properties of dichloromethane and butanol fractions from the stem bark of Broussonetia papyrifera . J. Korean Soc. Appl. Biol. Chem. 53, 297–303 (2010). https://doi.org/10.3839/jksabc.2010.046

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.3839/jksabc.2010.046

Key words

  • anti-inflammatory
  • apoptosis
  • Broussonetia papyrifera
  • nitrite oxidation
  • RT-PCR