Skip to main content
Applied Biological Chemistry
Download PDF

Inhibitory effect of populoside from the bark of Populus nigra on aldose reductase

Journal of the Korean Society for Applied Biological Chemistry volume 53, pages 729–733 (2010)Cite this article

Abstract

Bark extract of Populus nigra was examined as a possible inhibitor of aldose reductase, a key enzyme involved in diabetic complications. The aqueous acetone extract of P. nigra was subjected to gel filtration chromatography to produce an active compound, populoside, which inhibited aldose reductase with 50% inhibitory value of 18.55 μM in a non-competitive manner. Populoside also inhibited sorbitol accumulation by 48.84% at 50 μM. These results suggested that the P. nigra-derived component populoside has the potential to be used in the treatment of diabetic complications.

References

  • Gabbay KH (1973) The sorbitol pathway and the complications of diabetes. N Engl J Med 288, 831–836.

    Article  CAS  Google Scholar 

  • Jerkovic I and Mastelic J (2003) Volatile compounds from leafbuds of Populus nigra L (Salicaceae). Phytochemistry 63, 109–113.

    Article  CAS  Google Scholar 

  • Kador PF, Kinoshita JH, and Sharpless NE (1986) The aldose reductase inhibitor site. Metabolism 35, 109–113.

    Article  CAS  Google Scholar 

  • Kador PF (1988) The role of aldose reductase in the development of diabetic complications. Med Res Rev 8, 325–352.

    Article  CAS  Google Scholar 

  • Kato K, Nakayama K, Mizota M, Miwa I, and Okuda J (1991) Properties of novel aldose reductase inhibitors, M16209 and M16287, in comparison with known inhibitors, ONO-2235 and sorbinil. Chem Pharm Bull (Tokyo) 39, 1540–1545.

    Article  CAS  Google Scholar 

  • Kaul CL and Ramarao P (2001) The role of aldose reductase inhibitors in diabetic complications: recent trends. Methods Find Exp Clin Pharmacol 23, 465–475.

    Article  CAS  Google Scholar 

  • Kinoshita JH (1974) Mechanisms initiating cataract formation. Invest Ophthalmol 13, 713–724.

    CAS  Google Scholar 

  • Komoda Y (1989) Isolation of flavonoids from Populus nigra as delta 4-3-ketosteroid (5 alpha) reductase inhibitors. Chem Pharm Bull (Tokyo) 37, 3128–3130.

    Article  CAS  Google Scholar 

  • Kwon DJ, Bae YS, Si CL, Kim JK, Bae YS, and Li SM (2009) Phenolic compounds in the leaves of Populus ussuriensis and their antioxidant activities. Planta Med 75, 1165–1167.

    Article  Google Scholar 

  • Lee YS, Kang YH, Jung JY, Kang IJ, Han SN, Chung JS, Shin, HK, and Lim SS (2008) Inhibitory constituents of aldose reductase in the fruiting body of Phellinus linteus. Biol Pharm Bull 31, 765–768.

    Article  CAS  Google Scholar 

  • Malone JI, Knox G, Benford S, and Tedesco TA (1980) Red blood cell sorbitol: an indicator of diabetic control. Diabetes 29, 861–864.

    Article  CAS  Google Scholar 

  • Oates PJ and Mylari BL (1999) Aldose reductase inhibitors: therapeutic implications for diabetic complications. Expert Opin Investig Drugs 8, 2095–2119.

    Article  CAS  Google Scholar 

  • Oehlke J, Brudel M, and Blasig IE (1994) Benzoylation of sugars, polyols and amino acids in biological samples for high performance liquid chromatographic analysis. J Chromatogr B Biomed Sci Appl 655, 105–111.

    Article  CAS  Google Scholar 

  • Pearl IA and Darling SF (1970) Phenolic extractives of Salix Purpurea bark. Phytochemistry 9, 1277–1281.

    Article  CAS  Google Scholar 

  • Si CL, Li SM, Liu Z, Kim JK, and Bae YS (2009a) Antioxidant phenolic glycosides from the bark of Populus ussuriensis Kom. Nat Prod Res 6, 1–6.

    Google Scholar 

  • Si CL, Wu L, and Zhu ZY (2009b) Phenolic glycosides from Populus davidiana bark. Biochem Syst Ecol 37, 221–224.

    Article  CAS  Google Scholar 

  • Thieme H and Benecke R (1969) Isolation and determination of the constitution of cyclolignanglycoside from the leaves of Populus nigra L. {Pharmazie} 24, 567–572.

    CAS  Google Scholar 

  • Tomlinson DR, Willars GB, and Carrington AL (1992) Aldose reductase inhibitors and diabetic complications. Pharmacol Ther 54, 151–194.

    Article  CAS  Google Scholar 

  • Ueda H, Kawanishi K, and Moriyasu M (2004) Effects of ellagic acid and 2-(2,3,6-trihydroxy-4-carboxyphenyl) ellagic acid on sorbitol accumulation in vitro and in vivo. Biol Pharm Bull 27, 1584–1587.

    Article  CAS  Google Scholar 

  • Vertommen J, Rillaerts E, Gysels M, and De Leeuw I (1987) Erythrocyte sorbitol content in diabetic patients: relation to metabolic control. Diabete Metab 13, 182–186.

    CAS  Google Scholar 

  • Wang X, Wang Q, Xu G, and Xu L (1999) Advances in the research of constituents and pharmacology of Populus L. Nat Prod Res Dev 11, 65–74.

    CAS  Google Scholar 

  • Ward WH, Cook PN, Mirrlees DJ, Brittain DR, Preston J, Carey F, Tuffin DP, and Howe R (1991) (2,6-Dimethylphenylsulphonyl) nitromethane: a new structural type of aldose reductase inhibitor which follows kinetics and uses an allosteric binding site. Biochem Pharmacol 6, 2115–2123.

    Article  Google Scholar 

  • Zhang XF, Li X, Min BS, and Bae KH (2008) A new diterpenoid from the stem bark of Populus davidiana. Chin Chem Lett 19, 1080–1082.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, 200-702, Chuncheon, Republic of Korea

    Yeon Sil Lee & Soon Sung Lim

  2. Department of Agriculture, Yanbian University, 133000, Yan Ji, Jilin Prov, China

    Cheng-Bi Cui

  3. Institute of Natural Medicine, Hallym University, 200-702, Chuncheon, Republic of Korea

    Jin Kyu Kim, Jae-Yong Lee & Soon Sung Lim

  4. Departement of Forest Biomaterials Engineering, Kangwon National University, 200-701, Chuncheon, Republic of Korea

    Young Soo Bae

  5. Depratment of Biochemistry, Hallym University, 200-702, Chunchenon, Republic of Korea

    Jae-Yong Lee

  6. Department of Food Science and Nutrition, Hallym University, 200-702, Chunchenon, Republic of Korea

    Il-Jun Kang & Soon Sung Lim

Authors
  1. Yeon Sil Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cheng-Bi Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin Kyu Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Young Soo Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jae-Yong Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Il-Jun Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Soon Sung Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Soon Sung Lim.

Additional information

YSL and C-BC contributed equally.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lee, Y.S., Cui, CB., Kim, J.K. et al. Inhibitory effect of populoside from the bark of Populus nigra on aldose reductase. J. Korean Soc. Appl. Biol. Chem. 53, 729–733 (2010). https://doi.org/10.3839/jksabc.2010.110

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.3839/jksabc.2010.110

Key words

  • aldose reductase
  • diabetic complications
  • populoside
  • Populus nigra