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α-amylase and α-glucosidase inhibitors isolated from Triticum aestivum L. sprouts

Journal of the Korean Society for Applied Biological Chemistry volume 55, pages 47–51 (2012)Cite this article

Abstract

Inhibitory activities of the methanol extract from Triticum aestivum L. sprouts were examined against α-amylase and α-glucosidase. The active constituents of T. aestivum were isolated by chromatographic techniques and identified as γ-aminobutyric acid and ferulic acid on the basis of IR and NMR. γ-Aminobutyric acid and ferulic acid showed the high inhibitory activities with IC50 values of 5.4±0.2 and 9.5±0.1 mM against α-amylase, and 1.4±0.4 and 4.9±0.3 mM against α-glucosidase. The methoxy group on the hydroxycinnamic acid of ferulic acid derivatives played important functions in the α-amylase and α-glucosidase inhibitory activities. Based on the IC50 values of nitrite-scavenging activity, ferulic acid (98±3.9 μg/mL) was the most effective constituent, followed by γ-aminobutyric acid (182± 4.2 μg/mL), sinapic acid (301±2.7 μg/mL), and p-coumaric acid (454±2.2 μg/mL). These results indicate that γ-aminobutyric acid and ferulic acid could be useful as preventative agents, and possibly therapeutic modalities for the treatment of metabolic diseases.

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Authors and Affiliations

  1. Department of Bioenvironmental Chemistry and Institute of Agricultural Science & Technology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Eun-Young Jeong, Kyoung-Shik Cho & Hoi-Seon Lee

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  1. Eun-Young Jeong
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  2. Kyoung-Shik Cho
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  3. Hoi-Seon Lee
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Correspondence to Hoi-Seon Lee.

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Jeong, EY., Cho, KS. & Lee, HS. α-amylase and α-glucosidase inhibitors isolated from Triticum aestivum L. sprouts. J Korean Soc Appl Biol Chem 55, 47–51 (2012). https://doi.org/10.1007/s13765-012-0008-1

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  • DOI: https://doi.org/10.1007/s13765-012-0008-1

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