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Biotransformation of 6-deoxo type brassinosteroids in yeast (Saccharomyces cerevisiae) WAT21 cells

Journal of the Korean Society for Applied Biological Chemistry volume 55, pages 363–366 (2012)Cite this article

Abstract

We recently demonstrated that non-plant cells, yeast WAT21, produce a steroidal plant hormone, castasterone. To understand how castasterone is generated in WAT21 cells, deuteriumlabeled (26, 28-2H6) 6-deoxoteasterone, 6-deoxotyphasterol and 6-deoxocastasterone were fed to WAT21 cells, and their metabolites were analyzed by gas chromatography-mass spectrometry. When [2H6]-6-deoxoteasterone was fed, [2H6]-6-deoxo-3-dehydroteasterone and [2H6]-6-deoxotyphasterol were identified as metabolites. When [2H6]-6-deoxotyphasterol was used, [2H6]-6-deoxoteasterone and [2H6]-6-deoxo-3-dehydroteasterone and [2H6]-6-deoxocastasterone were detected. When [2H6]-6-deoxocastasterone was added, [2H6]-castasterone was identified. Taken together, a biosynthetic sequence, 6-deoxoteasterone↔6-deoxo-3-dehydroteasterone↔6-deoxotyphasterol→6-deoxocastasterone→castasterone seems to function to produce brassinosteroids in WAT21 cells. Coupled with the presence of a biosynthetic sequence, teasterone→3-dehydroteasterone↔typhasterol →castasterone, this suggests that brassinosteroids in WAT21 cells were biosynthesized via the same kind of pathways established in plants. This study provides a clue for possible mass-production of brassinosteroids in non-plant cells, yeast WAT21 for commercial use in agriculture.

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  1. Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea

    Seung-Hyun Son, Min-Kyun Kim & Seong-Ki Kim

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  1. Seung-Hyun Son
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  2. Min-Kyun Kim
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Correspondence to Seong-Ki Kim.

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Son, SH., Kim, MK. & Kim, SK. Biotransformation of 6-deoxo type brassinosteroids in yeast (Saccharomyces cerevisiae) WAT21 cells. J Korean Soc Appl Biol Chem 55, 363–366 (2012). https://doi.org/10.1007/s13765-012-1005-0

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