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Mango (Mangifera indica L.) peel extracts inhibit proliferation of HeLa human cervical carcinoma cell via induction of apoptosis

Journal of the Korean Society for Applied Biological Chemistry volume 55, pages 397–405 (2012)Cite this article

An Erratum to this article was published on 30 June 2012

Abstract

Flesh and peel extracts of five mango varieties were compared for their antioxidant and antiproliferative activities. Fozli peel (FP) extract was found to have the highest antioxidant activity and to significantly inhibit the proliferation of HeLa human cervical carcinoma cells in a dose-dependent manner. Hoechst 33342 staining revealed apoptotic nuclei with condensed chromatin, and cell growth arrest was confirmed by flow cytometry. Apoptotic signaling induced by FP extract was characterized by an increased Bax/Bcl-2 ratio and down-regulation of Bcl-2. Moreover, FP extract treatment triggered the proteolytic activation of caspases-3, -8, and -9 and the degradation of poly ADP-ribose polymerase in HeLa cells. These results indicate that FP is an excellent source of phenolic and/or flavonoid compounds, and may have applications in the treatment of human cervical carcinoma and as an antioxidant in the food and pharmaceutical industries. Gas chromatography-mass spectrometry analysis of FP extract revealed that 2,5-dihydroxyphenol was detected with highest amount and other compounds such as 5-hydroxymethyl-2-furancarboxaldehyde, pentadecanoic acid, (Z,Z)-9,12-octadecadienoic acid, and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one were also found in high amounts.

References

  • Ajila CM, Bhat SG, and Prasada Rao UJS (2007a) Valuable components of raw and ripe peels from two Indian mango varieties. Food Chem 102, 1006–1011.

    Article  CAS  Google Scholar 

  • Ajila CM, Naidu KA, Bhat SG, and Prasada Rao UJS. (2007b) Bioactive compounds and antioxidant potential of mango peel extract. Food Chem 105, 982–988.

    Article  CAS  Google Scholar 

  • Anuradha CD, Kanno S, and Hirano S (2000) Fluoride induces apoptosis by caspase-3 activation in human leukemia HL-60 cells. Arch Toxicol 74, 226–230.

    Article  CAS  Google Scholar 

  • Baydar NG, Ozkan G, and Yasar S (2007) Evaluation of the antiradical and antioxidant potential of grape extracts. Food Control 18, 1131–1136.

    Article  Google Scholar 

  • Bhutia SK, Mallick SK, Maiti S, Mishra D, and Maiti TK (2009) Abrus abrin derived peptides induce apoptosis by targeting mitochondria in HeLa cells. Cell Biol Int 33, 720–727.

    Article  CAS  Google Scholar 

  • Cha YY, Lee EO, Lee HJ, Park YD, Ko SG, Kim DH et al. (2004) Methylene chloride fraction of Scutellaria barbata induces apoptosis in human U937 leukemia cells via the mitochondrial signaling pathway. Clin Chim Acta 348, 41–48.

    Article  CAS  Google Scholar 

  • Dai J and Mumper RJ (2010) Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 15, 7313–7352.

    Article  CAS  Google Scholar 

  • Ghobrial IM, Witzig TE, and Adjei AA (2005) Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin 55, 178–194.

    Article  Google Scholar 

  • Green DR (2000) Apoptotic pathways: Paper wraps stone blunts scissors, Cell 102, 1–4.

    Article  CAS  Google Scholar 

  • Ha YL, Storkson J, and Pariza MW (1990) Inhibition of benzo(a)pyreneinduced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res 50, 1097–1101.

    CAS  Google Scholar 

  • Herr I and Debatin KM (2001) Cellular stress response and apoptosis in cancer therapy. Blood 98, 2603–2614.

    Article  CAS  Google Scholar 

  • Hong MY, Chapkin RS, Davidson LA, Turner ND, Morris JS, Carroll RJ et al. (2003) Fish oil enhances targeted apoptosis during colon tumor initiation in part by downregulating Bcl-2. Nutr Cancer 46, 44–51.

    Article  CAS  Google Scholar 

  • Huang X, Dai J, Fournier J, Ali AM, Zhang Q, and Frenkel K (2002) Ferrous ion autoxidation and its chelation in iron-loaded human liver HepG2 cells. Free Radic Biol Med 32, 84–92.

    Article  CAS  Google Scholar 

  • Kim H, Moon JY, Kim H, Lee DS, Cho M, Choi HK et al. (2010) Antioxidant and antiproliferative activities of mango (Mangifera indica L.) flesh and peel. Food Chem 121, 429–436.

    Article  CAS  Google Scholar 

  • Kim Y, Brecht JK, and Talcott ST (2007) Antioxidant Phytochemical and fruit quality changes in mango (Mangifera indica L.) following hot water immersion and controlled atmosphere storage. Food Chem 105, 1327–1334.

    Article  CAS  Google Scholar 

  • Kumaraswamy MV and Satish S (2008) Free radical scavenging activity and lipoxygenase inhibition of Woodfordia fructicosa Kurz and Betula utilis Wall. Afr J Biotechnol 7, 2013–2016.

    Google Scholar 

  • Leaver MJ, Tocher DR, and Obach A (2006) Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues. Comp Biochem Physiol A 145, 258–267.

    Article  Google Scholar 

  • Leontowicz M, Gorinstein S, Leontowicz H, Krzeminski R, Lojek A, Katrich E et al. (2003) Apple and pear peel and pulp and their influence on plasma lipids and antioxidant potentials in rats fed cholesterol-containing diets. J Agric Food Chem 51, 5780–5785.

    Article  CAS  Google Scholar 

  • Li L, Abe Y, Kanagawa K, Usui N, Imai K, Mashino T et al. (2004) Distinguishing the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-OH radical quenching effect from the hydroxyl radical scavenging effect in the ESR spin-trapping method. Anal Chim Acta 512, 121–124.

    Article  CAS  Google Scholar 

  • Liu SC, Lin JT, Wangc CK, Chen HY, and Yang DJ (2009) Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis Sonn.) flowers. Food Chem 114, 577–581.

    Article  CAS  Google Scholar 

  • Maisuthisakul P (2008) Antiradical scavenging activity and polyphenolic compounds extracted from Thai mango seed kernels. As J Food Ag-Ind 1, 87–96.

    Google Scholar 

  • Marzo I and Naval J (2008) Bcl-2 family members as molecular targets in cancer therapy. Biochem Pharmacol 76, 939–946.

    Article  CAS  Google Scholar 

  • Moon JY, Kim H, Cho M, Choi HK, Kim YS, Ashik M et al. (2011) The chloroform fraction of guava (Psidium. cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis. Food Chem 125, 369–375.

    Article  CAS  Google Scholar 

  • Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, and Hara Y (1996) Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radic Biol Med 21, 895–902.

    Article  CAS  Google Scholar 

  • Pavlovic V, Cekic S, Kocic G, Sokolovic D, and Živkovic V (2007) Effect of Monosodium Glutamate on Apoptosis and Bcl-2/Bax Protein Level in Rat Thymocyte Culture. Physiol Res 56, 619–626.

    CAS  Google Scholar 

  • Pino JA, Mesa J, Muòoz Y, Marta MP and Marbot R (2005) Volatile components from mango (Mangifera indica L.) cultivars. J Agric Food Chem 53, 2213–2223.

    Article  CAS  Google Scholar 

  • Reddy Avula CP and Femandes G (2000) Effect of dietary vitamin E on apoptosis and proliferation of murine splenocytes. Nutr Res 20, 225–236.

    Article  Google Scholar 

  • Ribeiro SMR, Barbosa LCA, Queiroz JH, Knodler M, and Schieber A (2008) Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem 110, 620–626.

    Article  CAS  Google Scholar 

  • Straszewski-Chavez SL, Abrahams VM, and Mor G (2005) The Role of apoptosis in the regulation of trophoblast survival and differentiation during pregnancy. Endocr Rev 26, 877–897.

    Article  CAS  Google Scholar 

  • Sroka Z and Cisowski W (2003) Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol 41, 753–758.

    Article  CAS  Google Scholar 

  • Stefanis L (2005) Caspase-dependent and -independent neuronal death: Two distinct pathways to neuronal injury. Neuroscientist 11, 50.

    Article  CAS  Google Scholar 

  • Wang Y, Chuang Y, and Hsu H (2008) The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan. Food Chem 106, 277–284.

    Article  CAS  Google Scholar 

  • Wolfe KL and Liu RH (2003) Apple peels as a value-added food ingredient. J Agric Food Chem 51, 1676–1683.

    Article  CAS  Google Scholar 

  • Xu BJ and Chang SK (2007) A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci 72, S159–S166.

    Article  CAS  Google Scholar 

  • Zimmermann KC, Bonzon C, and Green DR (2001) The machinery of programmed cell death. Pharmacol Ther 92, 57–70.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, 690-756, Republic of Korea

    Md. Ramjan Ali, Moon Jeong Yong, Youn Chul Ryu & Somi Kim Cho

  2. Department of Biochemistry and Biotechnology, Khwaja Yunus Ali University, Sirajganj, Bangladesh

    Md. Ramjan Ali

  3. Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756, Republic of Korea

    Rajendra Gyawali & Somi Kim Cho

  4. Department of Pharmacy, Rajshahi University, Rajshahi, Bangladesh, 6205

    Ashik Mosaddik

Authors
  1. Md. Ramjan Ali
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  2. Moon Jeong Yong
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  3. Rajendra Gyawali
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  4. Ashik Mosaddik
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  5. Youn Chul Ryu
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  6. Somi Kim Cho
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Correspondence to Somi Kim Cho.

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Md. R. Ali and M. J. Yong contributed equally.

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Ali, M.R., Yong, M.J., Gyawali, R. et al. Mango (Mangifera indica L.) peel extracts inhibit proliferation of HeLa human cervical carcinoma cell via induction of apoptosis. J Korean Soc Appl Biol Chem 55, 397–405 (2012). https://doi.org/10.1007/s13765-012-1024-x

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  • DOI: https://doi.org/10.1007/s13765-012-1024-x

Keywords

  • antioxidant
  • apoptosis
  • gas chromatography-mass spectrometry
  • HeLa human cervical carcinoma cells
  • mango
  • phytochemicals