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Online high performance liquid chromatography (HPLC)-ABTS+ based assay and HPLC-electrospray ionization mass spectrometry analysis of antioxidant phenolic compounds in Salsola komarovii

Journal of the Korean Society for Applied Biological Chemistry volume 55, pages 317–321 (2012)Cite this article

Abstract

Online high performance liquid chromatography (HPLC)-2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+) based assay system for phenolic antioxidants in Salsola komarovii extracts was applied. HPLC elute was mixed with a stabilized solution of ABTS+ reagents, and the negative peaks, indicating the antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. HPLC with electrospray ionization mass spectrometry was used to identify the seven flavonoids and two phenolic amides in the S. komarovii extracts. The antioxidant compounds were identified as rutin, isoquercitrin, astragalin, and isorhamnetin.

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Authors and Affiliations

  1. College of Pharmacy, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, 426-791, Republic of Korea

    Chul Young Kim

  2. Functional Food Center, KIST Gangneung Institute, Techno Valley, Gangneung, Gangwon-do, 210-340, Republic of Korea

    Hee Ju Lee, Cheol-Ho Pan & Chul Young Kim

  3. Wildlife Genetic Resources Center, National Institute of Biological Resources (NIBR), Gyungseo-dong, Seo-gu, Incheon, 404-708, Republic of Korea

    Eun-Sil Kim

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  1. Hee Ju Lee
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  2. Cheol-Ho Pan
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  3. Eun-Sil Kim
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Correspondence to Chul Young Kim.

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Lee, H.J., Pan, CH., Kim, ES. et al. Online high performance liquid chromatography (HPLC)-ABTS+ based assay and HPLC-electrospray ionization mass spectrometry analysis of antioxidant phenolic compounds in Salsola komarovii . J Korean Soc Appl Biol Chem 55, 317–321 (2012). https://doi.org/10.1007/s13765-012-1153-2

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