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Inhibition of xanthine oxidase by phenolic phytochemicals from Broussonetia papyrifera

Journal of the Korean Society for Applied Biological Chemistry volume 55, pages 587–594 (2012)Cite this article

Abstract

The roots of Broussonetia papyrifera were extracted into four different polar solvents: chloroform, 50% ethanol in water, ethanol, and water. The ethanol extract showed the most potent inhibition (72.3% at 20 g/mL) against xanthine oxidase (XOD). Chromatography of EE yielded nine phenolic phytochemicals, which were confirmed as broussochalcone A (1), broussochalcone B (2), 3,4-dihydroxyisolonchocarpin (3), 4-hydroxyisolonchocarpin (4), 3-′(3-methylbut-2-enyl)-3′,4′,7-trihydroxyflavane (5), kazinol A (6), kazinol B (7), kazinol E (8), and broussoflavan A (9). All isolated compounds (19) possessed potent antioxidant activities against 2,2-diphenyl-l-picrylhydrazyl and 2,2′-azino-bis-ethylbenzthiazoline-6-sulfonic acid (ABTS) radicals with IC50 values ranging from 5.8 to 252.8M. Although most compounds exhibited potent inhibition with IC50 values ranging 0.6–164 M against XOD, compounds 1 and 3 were found to be the principal contributors to the XOD inhibition in ethanol extract. The analysis of K I and K IS values proved that the two most promising compounds (1 and 3), present at high concentrations in the root barks as analyzed by using high-performance liquid chromatography analysis, were reversible mixed type I inhibitors.

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Authors and Affiliations

  1. Division of Applied Life Science (BK21 program), IALS, Graduate School of Gyeongsang National University, Jinju, 660-701, Republic of Korea

    Hyung Won Ryu, Ji Hye Lee, Jae Eun Kang, Young Min Jin & Ki Hun Park

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  1. Hyung Won Ryu
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  2. Ji Hye Lee
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  3. Jae Eun Kang
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  4. Young Min Jin
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  5. Ki Hun Park
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Correspondence to Ki Hun Park.

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Ryu, H.W., Lee, J.H., Kang, J.E. et al. Inhibition of xanthine oxidase by phenolic phytochemicals from Broussonetia papyrifera . J Korean Soc Appl Biol Chem 55, 587–594 (2012). https://doi.org/10.1007/s13765-012-2143-0

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