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Purification and characterization of a β-N-acetylhexosaminidase from wheat bran and its applicability to biocontrol of Fusarium solani

Journal of the Korean Society for Applied Biological Chemistry volume 55, pages 729–735 (2012)Cite this article

Abstract

N-acetyl-β-d-hexosaminidase was purified from wheat bran and characterized. The purified enzyme showed two protein bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with molecular mass of 75 and 78 kDa. The enzyme exhibited optimum pH and temperature at 5.0 and 50°C, respectively. The enzyme was active on the substrates of p-nitrophenyl-N-acetyl-β-d-glucosaminide (pNP-GlcNAc) and p-nitrophenyl-N-acetyl-β-d-galactosaminide (pNP-GalNAc), whereas inactive on pNP-β-d-glucopyranoside, pNP-β-d-galactopyranoside, swollen chitin, and colloidal chitin, suggesting high substrate specificity. The enzyme activity for pNP-GlcNAc was stable at pH 3–6 and under 50°C. The K m , V max and K cat for pNP-GlcNAc were 0.014 mM, 0.05 μmol/min, and 3.01×106 min−1, respectively. The enzyme could be completely inhibited at 1–10 mM HgCl2 and AgNO3, suggesting that the intact thiol group is essential for activity. β-N-Acetylhexosaminidase from wheat bran could inhibit the conidial germination and digest the hyphae of Fusarium solani.

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Authors and Affiliations

  1. Glucosamine Saccharide Materials-National Research Laboratory (GSMNRL), Division of Applied Bioscience and Biotechnololgy, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Wan-Taek Ju, Woo-Jin Jung, Kil-Yong Kim & Ro-Dong Park

  2. Department of Plant Protection, Faculty of Agriculture and Forestry, Tay Nguyen University, Buon Ma Thuot, Vietnam

    Van Nam Nguyen

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  1. Wan-Taek Ju
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  2. Van Nam Nguyen
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  3. Woo-Jin Jung
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  4. Kil-Yong Kim
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Correspondence to Ro-Dong Park.

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Ju, WT., Nguyen, V.N., Jung, WJ. et al. Purification and characterization of a β-N-acetylhexosaminidase from wheat bran and its applicability to biocontrol of Fusarium solani . J Korean Soc Appl Biol Chem 55, 729–735 (2012). https://doi.org/10.1007/s13765-012-2161-y

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