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Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 Cell

Journal of the Korean Society for Applied Biological Chemistry volume 56, pages 687–694 (2013)Cite this article

Abstract

Total phenolic contents of different fractions of the Psidium cattleianum leaf extract and their antioxidant capacity against several free radicals were examined. Protective effect of the ethyl acetate fraction (EAF) on H2O2-induced DNA damage in HepG2 cells were also evaluated, and the phytochemical profile of EAF was analyzed using tandem mass spectrometry. EAF derived from the 80% methanol extract of the leaf contained a remarkable amount of polyphenol and showed high levels of DPPH and alkyl radical scavenging activity, promoted cell viability, and protected against H2O2-induced DNA damage in HepG2 cells. Phytochemical analysis revealed that the major components in the EAF included quercetin monoglycoside, phloridizin, quercetin 3-diglycoside, quercetin-3-glucuronide, 2,6-dihydroxy-3,5-dimethyl-4-glucopyranosylbenzophenone, phenolic acid, guaijaverin, and naringin. The present study suggests possible synergistic or competitive antioxidant action of the major compounds of cattley guava leaf on H2O2-induced DNA damage in HepG2 cells. These results indicate that the ethyl acetate fraction of the guava leaf could be used as a potential source of natural antioxidants, and these findings will facilitate the utilization of guava leaf as a source of functional food.

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Authors and Affiliations

  1. Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, 690-756, Republic of Korea

    Jeong Yong Moon & Somi Kim Cho

  2. Department of Food Science and Engineering, Ewha Womans University, Seoul, 120-750, Republic of Korea

    Sarah Lee

  3. Korea Food Research Institute, Gyeonggi-do, 463-746, Republic of Korea

    Seongweon Jeong & Jong-Chan Kim

  4. Department of Oriental Pathology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong Dongdaemun-Gu, Seoul, 130-701, Republic of Korea

    Kwang Seok Ahn

  5. Pharmacy Department, BRAC university, Dkhaka, Bangladesh, 1212

    Ashik Mosaddik

  6. Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756, Republic of Korea

    Somi Kim Cho

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  1. Jeong Yong Moon
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  2. Sarah Lee
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  3. Seongweon Jeong
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  4. Jong-Chan Kim
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  7. Somi Kim Cho
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Correspondence to Somi Kim Cho.

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Moon, J.Y., Lee, S., Jeong, S. et al. Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 Cell. J Korean Soc Appl Biol Chem 56, 687–694 (2013). https://doi.org/10.1007/s13765-013-3156-z

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