Skip to main content
Applied Biological Chemistry
Download PDF

Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste

Journal of the Korean Society for Applied Biological Chemistry volume 57, pages 341–346 (2014)Cite this article

Abstract

Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii (AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at 750 mg L−1 phenol. The strain showed complete degradation of 750 mg L−1 phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of 28 mg L−1h−1. The doubling time was recorded approximately as 12.49 h−1. The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol.

References

  • Agarry SE, Durojaiye AO, and Solomon BO (2008) Microbial degradation of phenols: A review. Int J Environ Pollut 32, 12–28.

    Article  CAS  Google Scholar 

  • Ahmed I, Yokota A, and Fujiwara T (2007) A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth. Extremophiles 11, 217–224.

    Article  CAS  Google Scholar 

  • Arai H, Akahira S, Ohishi T, Maeda M, and Kudo T (1998) Adaptation of Comamonas testosteroni TA441 to utilize phenol: organization and regulation of the genes involved in phenol degradation. Microbiol 144(10), 2895–2903.

    Article  CAS  Google Scholar 

  • ATSDR (2011) Toxico-logical profile for phenol. U.S. Department of Health and Human Services, USA.

    Google Scholar 

  • Bandhyopadhyay K, Das D, Bhattacharyya P, and Maiti BR (2001) Reaction engineering studies on biodegradation of phenol by Pseudomonas putida MTCC 1194 immobilized on calcium alginate. Biochem Eng J 8, 179–186.

    Article  CAS  Google Scholar 

  • Banerjee A and Ghoshal AK (2010) Phenol degradation by Bacillus cereus: pathway and kinetic modeling. Bioresour Technol 101, 5501–5507.

    Article  CAS  Google Scholar 

  • Cao JW, Dong CM, Cao HB, and Shao ZZ (2011) Isolation of phenoldegrading bacteria from coking wastewater and their degradation gene. Huan Jing Ke Xue 32, 560–566.

    CAS  Google Scholar 

  • Carbajo JB, Boltes K, and Leton P (2010) Treatment of phenol in an anaerobic fluidized bed reactor (AFBR): continuous and batch regime. Biodegr 21, 603–613.

    Article  CAS  Google Scholar 

  • Carmona M, Lucas AD, Valverde JL, Velasco B, and Rodríguez JF (2006) Combined adsorption and ion exchange equilibrium of phenol on Amberlite IRA-420. Chem Eng J 117, 155–160.

    Article  CAS  Google Scholar 

  • Collins G, Foy C, McHugh S, and O’Flaherty V (2005) Anaerobic treatment of 2,4,6-trichlorophenol in an expanded granular sludge bed-anaerobic filter (EGSB-AF) bioreactor at 15 degrees C. FEMS Microbiol Ecol 53, 167–178.

    Article  CAS  Google Scholar 

  • Das S and Santra S (2012) Phenol removal from coal refining wastewater by immobilized cyanobacteria. Elect J Environ Agr Food Chem 11, 673–679.

    CAS  Google Scholar 

  • EEC (1980) Council Directive 80/778/EEC of 15 July 1980 relating to the quality of water intended for human consumption. Official Journal of the European Communities No.L 229, 30.08.1980, 11, France.

    Google Scholar 

  • El-Naas MH, Al-Muhtaseb SA, and Makhlouf S (2009) Biodegradation of phenol by Pseudomonas putida immobilized in polyvinyl alcohol (PVA) gel. J Hazard Mater 164, 720–725.

    Article  CAS  Google Scholar 

  • El-Sayed WS, Ibrahim MK, Abu-Shady M, El-Beih F, Ohmura N, Saiki H et al. (2003) Isolation and characterization of phenol-catabolizing bacteria from a coking plant. Biosci Biotechnol Biochem 67, 2026–2029.

    Article  CAS  Google Scholar 

  • Fialov A, Boschke E, and Bley T (2004) Rapid monitoring of the biodegradation of phenol-like compounds by the yeast Candida maltosa using BOD measurements. Int Biodeterior Biodegr 54, 69–76.

    Article  Google Scholar 

  • Gonzalez G, Herrera G, Garcia MT, and Pena M (2001) Biodegradation of phenolic industrial wastewater in a fluidized bed bioreactor with immobilized cells of Pseudomonas putida. Bioresour Technol 80, 137–142.

    Article  CAS  Google Scholar 

  • Hamitouche A-E, Bendjama Z, Amrane A, Kaouah F, and Hamane D (2012) Relevance of the Luong model to describe the biodegradation of phenol by mixed culture in a batch reactor. Ann Microbiol 62, 581–586.

    Article  CAS  Google Scholar 

  • Kotresha D and Vidyasagar GM (2008) Isolation and characterisation of phenol-degrading Pseudomonas aeruginosa MTCC 4996. World J Microbiol Biotech 24, 541–547.

    Article  CAS  Google Scholar 

  • Lin J, Reddy M, Moorthi V, and Qoma BE (2008) Bacterial removal of toxic phenols from an industrial effluent. Afr J Biotech 7, 2232–2238.

    CAS  Google Scholar 

  • Lowry M, Nagamani A, Sreenivasulu K, and Soligalla R (2009) Isolation and Characterization of Phenol-Degrading Soil Bacterium Xanthobacter flavus. Bioremed J 13, 1–6.

    Article  CAS  Google Scholar 

  • Mohite BV, Jalgaonwala RE, Pawar SD, and Morankar A (2010) Isolation and characterization of phenol degrading bacteria from oil contaminated soil. Innov Romanian Food Biotech 7, 61–65.

    CAS  Google Scholar 

  • Nuhoglu A and Yalcin B (2005) Modelling of phenol removal in a batch reactor. Process Biochemistry 40, 1233–1239.

    Article  CAS  Google Scholar 

  • Park JS, Brown MT, and Han T (2012) Phenol toxicity to the aquatic macrophyte Lemna paucicostata. Aquat Toxicol 106-7, 182–188.

    Article  Google Scholar 

  • Patel JB (2001) 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Mol Diagn 6, 313–321.

    Article  CAS  Google Scholar 

  • Pishgara R, Najafpour GD, Mousavi N, Bakhshi Z, and Khorrami M (2012) Phenol Biodegradation Kinetics in the Presence of Supplimentary Substrate. IJE Transc B: Applic 25, 181–191.

    Article  Google Scholar 

  • Polymenakou PN and Stephanou EG (2005) Effect of temperature and additional carbon sources on phenol degradation by an indigenous soil Pseudomonad. Biodegradation 16, 403–413.

    Article  CAS  Google Scholar 

  • Ravikumar S, Parimala PS, and Gokulakrishnan R (2011) Biodegradation of phenolic compounds by using Halotolerant microbes. Int J Plant Ani Environ Sci 1, 38–45.

    CAS  Google Scholar 

  • Rehfuss M and Urban J (2005) Rhodococcus phenolicus sp. nov., a novel bioprocessor isolated actinomycete with the ability to degrade chlorobenzene, dichlorobenzene and phenol as sole carbon sources. Syst Appl Microbiol 28, 695–701.

    Article  CAS  Google Scholar 

  • Rocha LL, de Aguiar Cordeiro R, Cavalcante RM, do Nascimento RF, Martins SC, Santaella ST et al. (2007) Isolation and characterization of phenol-degrading yeasts from an oil refinery wastewater in Brazil. Mycopathologia 164, 183–188.

    Article  CAS  Google Scholar 

  • Saravanan P, Pakshirajan K, and Saha P (2008) Growth kinetics of an indigenous mixed microbial consortium during phenol degradation in a batch reactor. Bioresour Technol 99, 205–209.

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28, 2731–2739.

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, and Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.

    Article  CAS  Google Scholar 

  • Tripathi M, Vikram S, Jain RK, and Garg S (2011) Isolation and Growth Characteristics of Chromium(VI) and Pentachlorophenol Tolerant Bacterial Isolate from Treated Tannery Effluent for its Possible Use in Simultaneous Bioremediation. Indian J Microbiol 51, 61–69.

    Article  CAS  Google Scholar 

  • US EPA (2007) National Recommended Water Quality Criteria. U.S. Environmental Protection Agency Office of Water Washington, USA.

    Google Scholar 

  • Veeresh GS, Kumar P, and Mehrotra I (2005) Treatment of phenol and cresols in upflow anaerobic sludge blanket (UASB) process: a review. Water Res 39, 154–170.

    Article  CAS  Google Scholar 

  • Yemendzhiev H, Gerginova M, Krastanov A, Stoilova I, and Alexieva Z (2008) Growth of Trametes versicolor on phenol. J Ind Microbiol Biotechnol 35, 1309–1312.

    Article  CAS  Google Scholar 

  • Zaki S (2006) Detection of meta-and ortho-cleavage dioxygenases in bacterial phenol degraders. J Appl Sci Environ Manag 10, 75–81.

    Google Scholar 

  • Zhai Z, Wang H, Yan S, and Yao J (2012) Biodegradation of phenol at high concentration by a novel bacterium: Gulosibacter sp. YZ4. J Chem Tech Biotech 87, 105–111.

    Article  CAS  Google Scholar 

  • Zhang Y, Lu DB, Ju TZ, Wang LT, Lin SX, Zhao Y et al. (2013) Biodegradation of Phenol Using Bacillus cereus WJ1 and Evaluation of Degradation Efficiency Based on a Graphene-Modified Electrode. Int J Electochem Sci 8, 504–519.

    CAS  Google Scholar 

  • Zhu X, Tian J, and Chen L (2012) Phenol degradation by isolated bacterial strains: kinetics study and application in coking wastewater treatment. J Chem Tech Biotech 87, 123–129.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. PARC Institute of Advanced Studies in Agriculture (PIASA), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Nazir Ahmad, Iftikhar Ahmed, Armghan Shahzad, Farrakh Mehboob & Ghulam Muhammad Ali

  2. National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Nazir Ahmad, Iftikhar Ahmed, Armghan Shahzad & Ghulam Muhammad Ali

  3. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Nauman Khalid

  4. Ecotoxicology Labs, National Institute for Bioremediation (NIB), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Farrakh Mehboob & Karam Ahad

Authors
  1. Nazir Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iftikhar Ahmed
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Armghan Shahzad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nauman Khalid
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Farrakh Mehboob
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Karam Ahad
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ghulam Muhammad Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Iftikhar Ahmed.

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ahmad, N., Ahmed, I., Shahzad, A. et al. Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste. J Korean Soc Appl Biol Chem 57, 341–346 (2014). https://doi.org/10.1007/s13765-013-4045-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13765-013-4045-1

Keywords

  • biodegradation
  • bioremediation
  • industrial waste
  • phenol
  • Pseudomonas
  • 16S rRNA gene