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Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste

Journal of the Korean Society for Applied Biological Chemistry volume 57, pages 341–346 (2014)Cite this article

Abstract

Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii (AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at 750 mg L−1 phenol. The strain showed complete degradation of 750 mg L−1 phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of 28 mg L−1h−1. The doubling time was recorded approximately as 12.49 h−1. The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol.

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Authors and Affiliations

  1. PARC Institute of Advanced Studies in Agriculture (PIASA), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Nazir Ahmad, Iftikhar Ahmed, Armghan Shahzad, Farrakh Mehboob & Ghulam Muhammad Ali

  2. National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Nazir Ahmad, Iftikhar Ahmed, Armghan Shahzad & Ghulam Muhammad Ali

  3. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Nauman Khalid

  4. Ecotoxicology Labs, National Institute for Bioremediation (NIB), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Farrakh Mehboob & Karam Ahad

Authors
  1. Nazir Ahmad
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  2. Iftikhar Ahmed
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  3. Armghan Shahzad
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  4. Nauman Khalid
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  5. Farrakh Mehboob
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  6. Karam Ahad
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  7. Ghulam Muhammad Ali
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Correspondence to Iftikhar Ahmed.

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Ahmad, N., Ahmed, I., Shahzad, A. et al. Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste. J Korean Soc Appl Biol Chem 57, 341–346 (2014). https://doi.org/10.1007/s13765-013-4045-1

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  • DOI: https://doi.org/10.1007/s13765-013-4045-1

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