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Differential role for BiP3 in rice immune receptor-mediated resistance
Journal of the Korean Society for Applied Biological Chemistry volume 57, pages539–542 (2014)
Endoplasmic reticulum-bound chaperone luminal-binding protein 3 (BiP3) has been found to regulate the immunity mediated by the membrane-bound extracellular immune receptors Xa3/Xa26 and Xa21, that encode non-arginine-aspartate (non-RD) kinases, against the bacterial pathogen Xanthomonas oryzae pathovar oryzae (Xoo). In contrast, BiP3 appeared not to regulate the immunity mediated by the intracellular immune receptor Pi5, which encodes a nucleotide-binding domain and leucine-rich repeat (NB-LRR) protein, against the fungal pathogen Magnaporthe oryzae. To further examine this differential role for BiP3 in rice immunity, we generated transgenic rice plants overexpressing BiP3 in the background of the NB-LRR intracellular immune receptor Xa1 that confers resistance to Xoo. Our molecular genetic and phenotype analyses revealed that BiP3 overexpression does not affect Xa1-mediated rice resistance to Xoo. Our current results thus provide evidence that BiP3 regulates membrane-bound non-RD kinase-mediated, but not the intracellular NB-LRR-mediated, rice immune responses and that its function does not depend on the type of pathogen.
Boller T and Felix G (2009) A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu Rev Plant Biol 60, 379–406.
Caplan JL, Zhu X, Mamillapalli P, Marathe R, Anandalakshmi R, and Dinesh-Kumar SP (2009) Induced ER chaperones regulate a receptor-like kinase to mediate antiviral innate immune response in plants. Cell Host Microbe 6, 457–69.
Chen DH and Ronald PC (1999) A rapid DNA minipreparation method suitable for AFLP and other PCR applications. Plant Mol Biol Rep 17, 53–7.
Dardick C and Ronald PC (2006) Plant and animal pathogen recognition receptors signal through non-RD kinases. PLoS Pathog 2, e2.
Han M, Ryu HS, Kim CY, Park DS, Ahn YK, and Jeon JS (2013) OsWRKY30 is a transcription activator that enhances rice resistance to the Xanthomonas oryzae pathovar oryzae. J Plant Biol 56, 258–65.
Jones JD and Dangl JL (2006) The plant immune system. Nature 444, 323–9.
Li J, Zhao-Hui C, Batoux M, Nekrasov V, Roux M, Chinchilla D et al. (2009) Specific ER quality control components required for biogenesis of the plant innate immune receptor EFR. Proc Natl Acad Sci USA 106, 15973–8.
Liu J, Liu X, Dai L, and Wang GL (2007) Recent progress in elucidating the structure, function and evolution of disease resistance genes in plants. J Genet Genomics 34, 765–76.
Martin GB, Bogdanove AJ, and Sessa G (2003) Understanding the functions of plant disease resistance proteins. Annu Rev Plant Biol 54, 23–61.
Nekrasov V, Li J, Batoux M, Chu ZH, Lacombe S, Rougon A et al. (2009) Control of the pattern-recognition receptor EFR by and ER protein complex in plant immunity. EMBO J 28, 3428–38.
Padmanabhan MS and Dinesh-Kumar SP (2010) All hands on deck-the role of chloroplasts, endoplasmic reticulum, and the nucleus in driving plant innate immunity. Molecular Plant-Microbe Interactions 23, 1368–80.
Park CJ, Bart R, Chern M, Canlas PE, Bai W, and Ronald PC (2010) Overexpression of the endoplasmic reticulum chaperone BiP3 regulates XA21-mediated innate immunity in rice. PLoS One 5, e9262.
Park CJ, Song MY, Kim CY, Jeon JS, and Ronald PC (2014) Rice BiP3 regulates immunity mediated by the PRRs XA3 and XA21 but not immunity mediated by the NB-LRR protein, Pi5. Biochem Biophys Res Commun 448, 70–5.
Saijo Y (2010) ER quality control of immune receptors and regulators in plants. Cell Microbiol 12, 716–24.
Song W, Wang G, Chen L, Kim H, Pi L, Holsten T et al (1995) A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science 270, 1804–6.
Sun X, Cao Y, Yang Z, Xu C, Li X, Wang S et al. (2004) Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. Plant J 37, 517–27.
Yoshimura S, Yamanouchi U, Katayose Y, Toki S, Wang ZX, Kono I et al. (1998) Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation. Proc Natl Acad Sci USA 95, 1663–8.
About this article
- endoplasmic reticulum-bound chaperone luminal-binding protein 3
- nucleotide-binding domain and leucine-rich repeat
- Xanthomonas oryzae pathovar oryzae