Skip to main content
  • Article
  • Published:

Electron beam irradiation and dilute alkali pretreatment for improving saccharification of rice straw

Abstract

Rice straw is one of the most abundant and low-cost biomasses available in the world. Thus, the rice straw as a potential candidate for future energy and chemical resource has been intensively studied in order to use as the current fossil fuels. However, the structure of rice straw makes it difficult to hydrolyze into fermentable sugars owing to the cellulose in rice straw being tightly surrounded by hemicellulose and lignin, thus pretreatment of rice straw is needed for this process. In the present study, an alkali pretreatment method assisted by electron beam irradiation was investigated to improve the saccharification in an enzymatic hydrolysis yield. After pretreatment, cellulose in rice straw was increased from 39.5 to 71.1%, and lignin decreased from 19.5 to 6.4%. The sugar yield of the pretreated rice straw increased with an increase in irradiation dose. The results of XRD and Fourier transform infrared spectroscopy analyses showed that the properties of the straw were changed by this pretreatment, which favored the following enzymatic hydrolysis.

References

  • Agbor VB, Cicek N, Sparling R, Berlin A, and Levin DB. (2011) Biomass pretreatment: Fundamentals toward application. Biotechnol Adv 29, 675–85.

    Article  CAS  Google Scholar 

  • Bak JS, Ko JK, Han YH, Lee BC, Choi IG, and Kim KH. (2009) Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment. Bioresour Technol 100, 1285–90.

    Article  CAS  Google Scholar 

  • Binod P, Satyanagalakshmi K, Sindhu R, Janu KU, Sukumaran RK, and Pandey A. (2012) Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse. Renew Energy 37, 109–16.

    Article  CAS  Google Scholar 

  • Binod P, Sindhu R, Singhania RR, Vikram S, Devi L, Nagalakshmi S, Kurien N, Sukumaran RK, and Pandey A. (2010) Bioethanol production from rice straw: An overview. Bioresour Technol 101, 4767–74.

    Article  CAS  Google Scholar 

  • Chung BY, Lee JT, Lee SS, and Kim UJ. (2012) A comparison of the efficiency of electron beam irradiation on enzymatic hydrolysis between 4 doses of 25 kGy and a single dose of 100 kGy for bioethanol production. J Korean Soc Appl Biol Chem. DOI 10.1007/s13765-012-2021-9.

    Google Scholar 

  • Hii KL, Yeap SP, and Mashitah. (2012) Pretreatment of pressed pericarp fibers(PPF) using alcohols as solvent to increase the accessibility of cellulose for cellulose production. J Korean Soc Appl Biol Chem 55, 507–14.

    Article  CAS  Google Scholar 

  • Janker-Obermeier I, Siebera V, Faulstich M, and Schieder D. (2012) Solubilization of hemicellulose and lignin from wheat straw through microwave-assisted alkali treatment. Ind Crop Prod 39, 198–203.

    Article  CAS  Google Scholar 

  • Jiang M, Zhao M, Zhou Z, Huang T, Chen X, and Wang Y. (2011) Isolation of cellulose with ionic liquid from steam exploded rice straw. Ind Crop Prod 33, 734–8.

    Article  CAS  Google Scholar 

  • Kang KE and Jeong GT. (2012) Pretreatment of rapeseed straw by soaking in aqueous ammonia. Bioprocess Biosyst Eng 35, 77–84.

    Article  CAS  Google Scholar 

  • Li MF, Fan YM, Xu F, Sun RC, and Zhang XL. (2010) Cold sodium hydroxide/urea based pretreatment of bamboo for bioethanol production: Characterization of the cellulose rich fraction. Ind Crop Prod 32, 551–9.

    Article  CAS  Google Scholar 

  • Liu J, Wang Q, Wang S, Zou D, and Sonomoto K. (2012) Utilisation of microwave-NaOH pretreatment technology to improve performance and L-lactic acid yield from vinasse. Biosyst Eng 112, 6–13.

    Article  Google Scholar 

  • Mood SH, Golfeshan AH, Tabatabaei M, Jouzani GS, Najafi GH, Gholami M, and Ardjmand M. (2013) Lignocellulosic biomass to bioethanol, acomprehensive review with a focus on pretreatment. Renew Sust Energ Rev 27, 77–93.

    Article  Google Scholar 

  • Niu K, Chen P, Zhang X, and Tan WS. (2009) Enhanced enzymatic hydrolysis of rice straw pretreated by alkali assisted with photocatalysis technology. J Chem Technol Biotechnol 84, 1240–5.

    Article  CAS  Google Scholar 

  • NREL. (2008) Enzymatic saccharification of lignocellulosic biomass. National Renewable Energy Laboratory, USA.

    Google Scholar 

  • NREL. (2012) Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory, USA.

    Google Scholar 

  • Pal S, Banik SP, and Khowala S. (2013) Mustard stalk and straw: A new source for production of lignocellulolytic enzymes by the fungus Termitomyces clypeatus and as a substrate for saccharification. Ind Crop Prod 41, 283–8.

    Article  CAS  Google Scholar 

  • Paraj JC, Alonso JL, and Vzquez D. (1994) Effect of selected operational variables on the susceptibility of NaOH-pretreated pine wood to enzymatic hydrolysis: a mathematical approach. Wood Sci Technol 28, 297–300.

    Google Scholar 

  • Park WS, Hwang MH, Kim TH, Lee MJ, and Kim IS. (2009) Enhancement in characteristics of sewage sludge and anaerobic treatability by electron beam pre-treatmen. Radiat Phys Chem 78, 124–9.

    Article  CAS  Google Scholar 

  • Poornejad N, Karimi K, and Behzad T. (2013) Improvement of saccharification and ethanol production from rice straw by NMMO and [BMIM][OAc] pretreatments. Ind Crop Prod 41, 408–13.

    Article  CAS  Google Scholar 

  • Sarkar N, Ghosh SK, Bannerjee S, and Aikat K. (2012) Bioethanol production from agricultural wastes: An overview. Renew Energy 37, 19–27.

    Article  CAS  Google Scholar 

  • Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, and Osborne J. (2009) A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresour Technol 98, 3000–11.

    Article  Google Scholar 

  • Sun RC, Tomkinson J, Ma PL, and Liang SF. (2000) Comparative study of hemicelluloses from rice straw by alkali and hydrogen peroxide treatments. Carbohydr Polym 42, 111–22.

    Article  CAS  Google Scholar 

  • Taherzadeh MJ and Karimi K. (2008) Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: A Review. Int J Mol Sci 9, 1621–51.

    Article  CAS  Google Scholar 

  • Talebnia F, Karakashev D, and Angelidaki I. (2010) Production of bioethanol from wheat straw: An overview on pretreatment, hydrolysis and fermentation. Bioresour Technol 101, 4744–53.

    Article  CAS  Google Scholar 

  • Technical Association of the Pulp and Industry. (2002) TAPPI standards and suggested methods, USA.

    Google Scholar 

  • Yang C, Tan T, and Zhu X. (2012) Adsorptive capacity of ethylenediamine treated oxidised rice straw for sulfur dioxide. Carbohydr Polym 87, 1843–8.

    Article  CAS  Google Scholar 

  • Yang L, Cao J, Mao J, and Jin Y. (2013) Sodium carbonate-sodium sulfite pretreatment for improving the enzymatic hydrolysis of rice straw. Ind ai]Crop Prod 43, 711–7.

    Article  CAS  Google Scholar 

  • Zhu S, Wu Y, Yu Z, Chen Q, Wu G, Yu F, Wang C, and Jin S. (2006) Microwave-assisted alkali pre-treatment of wheat straw and its enzymatic hydrolysis. Biosyst Eng 94, 437–42.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Joon-Pyo Jeun.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, DY., Lee, BM., Lee, JY. et al. Electron beam irradiation and dilute alkali pretreatment for improving saccharification of rice straw. J Korean Soc Appl Biol Chem 57, 591–595 (2014). https://doi.org/10.1007/s13765-014-4191-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13765-014-4191-0

Keywords