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A putative cold shock protein-encoding gene isolated from Arthrobacter sp. A2-5 confers cold stress tolerance in yeast and plants

Journal of the Korean Society for Applied Biological Chemistry volume 57, pages 775–782 (2014)Cite this article

An Erratum to this article was published on 10 February 2015

Abstract

A putative cold shock protein gene, designated as ArCspA, was isolated from Arthrobacter sp. A2-5 extracted from soil at the South Pole. The ArCspA gene is 873 nucleotide bp long and includes a 207-bp short open reading frame (ORF) with 49.3–92% amino acid identity to peptide sequences of other bacterial cold shock proteins. Northern blot analysis revealed that ArCspA was highly expressed at low temperatures. Bio-functional analysis using ArCspA-overexpressed transgenic Saccharomyces cerevisiae showed that ArCspA conferred cold tolerance on yeast at low temperatures (15°C). We then developed an ArCspA-overexpressed transgenic tobacco line to determine whether ArCspA is also functional in plants. After cold treatment at −25°C for 90 min followed by recovery for 4 weeks at 25°C, 17 transgenic lines survived at a high rate (60.0%), whereas under the same treatment conditions, wild-type plants did not survive. We also found that progeny of transgenic tobacco plants subjected to freezing stress at −20°C had significantly higher seed germination ability than wild-type plants. These results clearly indicate that the ArCspA protein plays an important role in cold tolerance in both yeast and plants.

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Authors and Affiliations

  1. Rural Development Administration, National Academy of Agricultural Science, Jeonju, 560-500, Republic of Korea

    Seong-Kon Lee, Sung-Han Park, Jeong-Won Lee, Hae-Min Lim, Sun-Young Jung, In-Cheol Park & Soo-Chul Park

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  1. Seong-Kon Lee
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  2. Sung-Han Park
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  3. Jeong-Won Lee
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  4. Hae-Min Lim
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  7. Soo-Chul Park
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Correspondence to Soo-Chul Park.

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Lee, SK., Park, SH., Lee, JW. et al. A putative cold shock protein-encoding gene isolated from Arthrobacter sp. A2-5 confers cold stress tolerance in yeast and plants. J Korean Soc Appl Biol Chem 57, 775–782 (2014). https://doi.org/10.1007/s13765-014-4238-2

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  • DOI: https://doi.org/10.1007/s13765-014-4238-2

Keywords

  • ArCspA gene
  • ArCspA transgenic tobacco
  • ArCspA transgenic yeast