Fig. 1

3T3L1 preadipocytes were treated with 20 μM quercetin (a) or 20 μM kaempferide (b) for the indicated times. Whole-cell lysates were prepared and immunoblot analysis was performed using anti-phospho adenosine monophosphate-activated protein kinase (AMPK) (Thr172). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control