Citrus unshiu leaf extract containing phytol as a major compound induces autophagic cell death in human gastric adenocarcinoma AGS cells

Applied Biological Chemistry

Table 1 Compounds identified from the methanolic extract of C. unshiu leaf by GC–MS

No.	RI^a	Name^b	Area %^c
1	923	Butyrolactone	0.47
2	933	1,2-Cyclopentadione	0.22
3	1,060	Furaneol	0.38
4	1,147	2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one	3.28
5	1,170	Benzoic acid	1.53
6	1,219	4-Vinylphenol	1.06
7	1,316	4-Ethenyl-2-methoxyphenol	13.81
8	1,363	p-Hydroxybenzaldehyde	1.17
9	1,394	β-Elemene	0.69
10	1,554	Elemol	1.25
11	1,568	3,4-Dimethoxyacetophenone	0.90
12	1,743	Coniferyl alcohol	0.34
13	1,919	Hexadecanoic acid, methyl ester	1.57
14	1,955	Hexadecanoic acid	6.58
15	1,964	Bis(2-methoxyethyl)-phthalate	0.18
16	2,054	Heptadecanoic acid	0.31
17	2,090	(Z,Z)-9,12-octadecadienoic acid methyl ester	0.42
18	2,109	(E)-Phytol	47.35
19	2,128	(Z,Z)-9,12-octadecadienoic acid	0.54
20	2,155	(Z)-9-Octadecenoic acid	0.47
21	2,398	Tetracosane	0.31
22	2,499	Pentacosane	0.31
23	2,507	Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester	1.23
24	2,551	Bis(2-ethylhexyl) phthalate	0.72
25	2,826	Squalene	0.70
26	3,000<	Vitamin E	2.18
27	3,000<	delta.5-Ergostenol	0.28
28	3,000<	Stigmasterol	0.29
29	3,000<	gamma-Sitosterol	0.91
30	3,000<	Nobiletin	0.15
		Total	89.60

^aRetention index
^bCompounds tentatively identified based on parent molecular ions, retention times, retention indices and elution order, and fragmented spectra compared with the literature
^cPeak area percentage (peak area relative to the total peak area %)