Table 2 List of proteins differentially expressed in soybean sprouts and identified by MALDI-TOF MS analysis
From: Biochemical and proteomic analysis of soybean sprouts at different germination temperatures
Spot no. | ACa | Protein name | Score | Expect | MPb | Theor. MWc | Theor.pI d | SC (%)e | Source | GO term |
|---|---|---|---|---|---|---|---|---|---|---|
1 | ITRA_SOYBN | Trypsin inhibitor A | 98 | 8.00E−05 | 13 | 24,275 | 4.99 | 29 | Glycine max | Serine-type endopeptidase inhibitor activity |
2 | ITRA_SOYBN | Trypsin inhibitor A | 75 | 0.018 | 14 | 24,275 | 4.99 | 32 | Glycine max | Serine-type endopeptidase inhibitor activity |
3 | C6SWW4 | Uncharacterized protein | 105 | 6.30E−05 | 9 | 22,718 | 4.85 | 39 | Glycine max | Endopeptidase inhibitor activity |
4 | GLCB_SOYBN | Beta-conglycinin, beta chain | 79 | 0.0,073 | 9 | 50,578 | 5.88 | 19 | Glycine max | Nutrient reservoir activity |
5 | GLCB_SOYBN | Beta-conglycinin, beta chain | 70 | 0.057 | 9 | 50,578 | 5.88 | 16 | Glycine max | Nutrient reservoir activity |
6 | B6EBD5 | SGT1-2 | 60 | 2.2 | 10 | 40,812 | 5.18 | 23 | Glycine max | None |
7 | K7KT39 | Uncharacterized protein | 34 | 7.10E+02 | 5 | 7,398 | 5.17 | 72 | Glycine max | None |
8 | K7MAJ1 | Uncharacterized protein | 49 | 23 | 4 | 7,387 | 5.69 | 83 | Glycine max | None |
9 | K7LI10 | Uncharacterized protein | 34 | 7.60E+02 | 9 | 72,269 | 6.56 | 17 | Glycine max | None |
10 | K7KHZ7 | Uncharacterized protein | 43 | 1.10E+02 | 4 | 11,627 | 4.81 | 18 | Glycine max | Zinc ion binding |
11 | K7MAX3 | Uncharacterized protein | 49 | 23 | 17 | 188,585 | 6.48 | 11 | Glycine max | None |
12 | C6TDW0 | Putative uncharacterized protein | 61 | 1.7 | 9 | 20,774 | 9.25 | 54 | Glycine max | Sequence-specific DNA binding transcription factor activity |
13 | GLCB_SOYBN | Beta-conglycinin, beta chain | 62 | 0.37 | 9 | 50,578 | 5.88 | 31 | Glycine max | Nutrient reservoir activity |
14 | GLCB_SOYBN | Beta-conglycinin, beta chain | 65 | 0.18 | 10 | 50,578 | 5.88 | 32 | Glycine max | Nutrient reservoir activity |
15 | K7MXA1 | Uncharacterized protein (Fragment) | 54 | 7.3 | 6 | 12,584 | Glycine max | Structural molecule activity | ||
16 | VSPB_SOYBN | Stem 31 kDa glycoprotein | 128 | 8.60E−08 | 15 | 29,433 | 6.72 | 61 | Glycine max | Acid phosphatase activity, nutrient reservoir activity |
17 | O22121 | Beta subunit of beta conglycinin (Fragment) | 72 | 0.13 | 11 | 47,947 | 5.67 | 27 | Glycine max | Nutrient reservoir activity |
18 | I1MIJ9 | Uncharacterized protein | 75 | 0.066 | 18 | 142,843 | 6.02 | 21 | Glycine max | ADP binding, defense response |
19 | I1MJU7 | Uncharacterized protein | 104 | 8.00E−05 | 17 | 73,880 | 5.2 | 25 | Glycine max | ATP binding, protein folding |
20 | I1JPC5 | Uncharacterized protein | 121 | 1.60E−06 | 17 | 67,939 | 4.98 | 39 | Glycine max | ATP binding, nucleotide binding |
21 | I1KPN3 | Uncharacterized protein | 135 | 6.30E−08 | 20 | 73,660 | 5.08 | 33 | Glycine max | ATP binding, nucleotide binding |
22 | O22121 | Beta subunit of beta conglycinin (Fragment) | 128 | 3.20E−07 | 17 | 47,947 | 5.67 | 43 | Glycine max | Nutrient reservoir activity |
23 | K7KDG0 | Uncharacterized protein | 27 | 4.20E+03 | 3 | 8,902 | Glycine max | None | ||
24 | GLCA_SOYBN | Beta-conglycinin, alpha chain | 84 | 0.0019 | 14 | 70,535 | 5.07 | 18 | Glycine max | Nutrient reservoir activity |
25 | Q9FZP9 | Alpha’ subunit of beta-conglycinin (Fragment) | 61 | 1.5 | 10 | 65,160 | 5.23 | 14 | Glycine max | Nutrient reservoir activity |
26 | Q9FZP9 | Alpha’ subunit of beta-conglycinin (Fragment) | 91 | 0.0017 | 14 | 65,160 | 5.23 | 21 | Glycine max | Nutrient reservoir activity |
27 | Q0MUU5 | Beta-conglycinin alpha’-subunit | 78 | 0.035 | 13 | 70,130 | 5.43 | 27 | Glycine max | Nutrient reservoir activity |
28 | GLCA_SOYBN | Beta-conglycinin, alpha chain | 90 | 0.00051 | 13 | 70,535 | 5.07 | 19 | Glycine max | Nutrient reservoir activity |
29 | O22120 | Alpha subunit of beta conglycinin (Fragment) | 133 | 1.00E−07 | 18 | 63,184 | 4.92 | 32 | Glycine max | Nutrient reservoir activity |
30 | GLCA_SOYBN | Beta-conglycinin, alpha chain | 118 | 8.60E−07 | 16 | 70,535 | 5.07 | 28 | Glycine max | Nutrient reservoir activity |
31 | C6SWV3 | Uncharacterized protein | 85 | 0.0069 | 13 | 27,847 | 5.72 | 49 | Glycine max | None |
32 | GLCB_SOYBN | Beta-conglycinin, beta chain | 71 | 0.042 | 12 | 50,578 | 5.88 | 25 | Glycine max | Nutrient reservoir activity |
33 | I1N887 | Uncharacterized protein | 56 | 5.3 | 10 | 34,958 | 7.72 | 47 | Glycine max | Chaperone |