Fig. 2
From: h-Prune as a novel binding protein of NS5A that regulates ERK1/2 activation
Domain analysis of NS5A-c interacting with h-prune. (A) The class II PxxP domain of the NS5A-c portion. Five proline amino acids in the NS5A-c PxxP motif were mutated to alanine. (B) A colony-lift filter assay with NS5A-F or NS5A-F-mut, and the full-length h-prune protein. NS5A-F bound to the h-prune protein but not to NS5A-F-mut. pHybLex/Zeo-Fos2 and pYESTrp-Jun were used for the positive control [(+) control], and pHybLex/Zeo-Lamin alone was transfected into the L 40 yeast strain for the negative control [(−) control]