Fig. 3
From: h-Prune as a novel binding protein of NS5A that regulates ERK1/2 activation
h-Prune consensus sequences in different species and their exopolyphosphatase activity. (A) h-Prune consensus sequences in different species. PPase consensus sequences among humans, yeast, and, Drosophila are depicted in gray. Invariant sequences in the prune DDH motifs are shaded in black with white letters. (B) SDS-PAGE of PolyP45 hydrolysis using purified h-prune. The h-prune protein with a His tag was purified from E. coli cells and mixed with 100 μM inorganic polyphosphate 45 (PolyP45) in reaction buffer. After 30 min or 1 h incubation at 37 °C, PolyP hydrolysates were subjected to PAGE on a 22 % polyacrylamide gel with 7 M urea. Gels were visualized after staining with 0.05 % toluidine blue. PolyP was time-dependently hydrolyzed when incubated with h-prune (arrow) (n = 4 experiments)