Fig. 5From: h-Prune as a novel binding protein of NS5A that regulates ERK1/2 activationh-Prune restores NS5A-mediated attenuation of Erk1/2 signaling. (A) Western blots for phospho-ERK1/2. Huh7 cells transfected with FLAG-NS5A and Myc-h-prune were treated with 20 ng/mL rhEGF and immunoblotted with anti-phospho ERK1/2 antibody. (B) Bars denote the density ratio of phospho-ERK1/2 relative to β-actin. All ratio values were normalized to the WT ratio at time 0 and defined as 1 (*P < 0.05, **P < 0.01, ***P < 0.001 vs. WT 0 time; #P < 0.05, ##P < 0.01, ns (not significant) versus mock at each time point; mean ± SE, n = 4 experiments)Back to article page