Table 1 Oligonucleotides used in this study
From: h-Prune as a novel binding protein of NS5A that regulates ERK1/2 activation
Primer name | Primer sequence | Vector |
|---|---|---|
A. PCR primers used in the construction of pHybLex/Zeo-NS5A-F, -F-A, -F-B, and -F–C | ||
 NS5A (Full-F) | 5′-GCGGCCGCTTTCCGGCTCGTGGCTAAGG-3′ | a |
 NS5A (Full-R) | 5′-GTCGACTCATTATCAGCAGCAGACGACGTCC-3′ | a |
 NS5A (150-R) | 5′-GCGGCCGCTTTTCACAGAATTGGATGGGGTGC-3′ | a |
 NS5A (300-F) | 5′-GCGGCCGCTTGCGGAGATCCTGCGGAGAT-3′ | a |
 NS5A (300-R) | 5′-GTCGACTCATTACGCCGGAACGGATACTTCCC-3′ | a |
B. PCR primers used in the construction of pHybLex/Zeo-NS5A-F-mut | ||
 NS5A (Full-F) | 5′-GCGGCCGCTTTCCGGCTCGTGGCTAAGG-3′ | a |
 NS5A (Full-R) | 5′-GTCGACTCATTATCAGCAGCAGACGACGTCC-3′ | a |
 NS5A-mut1 | 5′-ATCGTCCTCTTCCTCCGTGCGGCTGCTATCGCTGGGGCCTTGGTAGGT G-3′ | a |
 NS5A-mut2 | 5′-CACCTACCAAGGCCCCAGCGATAGCAGCCGCACGGAGGAAGAGGACGAT-3′ | a |
C. PCR primers used in the construction of pYESTrp2, pMAL, and pCMV-Tag1-h-prune | ||
 h-Prune-F | 5′-CCGGAT CCATGGAGGACTACCTGCAGGGT-3′ | b, c, d |
 h-Prune-R1 | 5′-CCCTCGAGCTTCTTGGACAGGGAGGC-3′ | b, d |
 h-Prune-R2 | 5′-CCGTCGACAGCTTCTTGGACAGGGAGGC-3′ | c |