Skip to main content
Applied Biological Chemistry

Table 1 Sequences of primers used for plasmid construction

From: Assessing bioavailability and genotoxicity of heavy metals and metallic nanoparticles simultaneously using dual-sensing Escherichia coli whole-cell bioreporters

Genes

Direction

Restriction enzyme site

Sequence (5′–3′)

recAp

Forward

BglII

GTAGATCTGCCACTGCCCGCGGTGAAGG

 

Reversed

–

ATGTATATCTCCTTCTTATACTTAACTAATATACTAAGATGCCGGGTAATACCGG

eGFP

Forward

–

CTTAGTATATTAGTTAAGTATAAGAAGGAGATATACATATGGTGAGCAAGGGCGAGG

 

Reversed

XhoI

ATCTCGAGTTACTTGTACAGCTCGTCCATGC

zntAp

Forward

BglII

ACAGATCTCGGCCTGCTACTTTGCC

 

Reversed

XbaI

CCTCTAGAGGGCTTTCTTGCCGTGAT

mCherry

Forward

BamHI

GTGGATCCATGGTGAGCAAGGGCGAG

 

Reversed

XhoI

ATCTCGAGCTTGTACAGCTCGTCCATGC

  1. Bold letters indicate the restriction enzyme sites
  2. The underlined italic letters indicate the ribosome binding sites
Back to article page