Anti-inflammatory effects of luteolin and luteoloside from Taraxacum coreanum in RAW264.7 macrophage cells

Applied Biological Chemistry

Table 1 Primers and conditions used in PCR

mRNA	Primer sequence	PCR conditions
NF-κB	F: GCA-GCC-TAT-CAC-CAA-CTC-T R: TAC-TCC-TTC–TTC-ACC-A	48 °C Cycle: 35
iNOS	F: CCT–CCT-CCA-CCC-TAC-CAA-GT	53 °C Cycle: 35
iNOS	R: CAC-CCA-AAG-TGC-CTC-AGT-CA	53 °C Cycle: 35
COX-2	F: AAG-ACT-TGC-CAG-GCT-GAA-CT	53 °C Cycle: 35
COX-2	R: CTT-CTG-CAG-TCC-AGG-TTC-AA	53 °C Cycle: 35
GAPDH	F: TCA-TGA-AGT-GTG-ACG-TTG-ACA-TCC-GT	60 °C Cycle: 35
GAPDH	R: CCT-AGA-AGC-ATT-TGC-GGT-GCA-CGA-TG	60 °C Cycle: 35

NF-κB Nuclear factor-kappa B, iNOS inducible nitric oxide synthase, COX-2 cyclooxygenase-2, GAPDH Glyceraldehyde 3-phosphate dehydrogenase