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Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Cloning of a novel endogenous promoter for foreign gene expression in Phaeodactylum tricornutum

Fig. 3

Cell growth (filled triangle) and relative eGFP fluorescence (filled square) of P. tricornutum transformed with the construct containing fcpA (A), CIP1 (B), GLNA-501 (C), and GLNA-997 promoter (D); statistical analysis on the eGFP expression level of each promoter at Day 6 (E) and Day 11 (F). Each transformed P. tricornutum was seeded with the concentration of 105 cell/mL at the day 0 and cultivated. Cell density and eGFP expression were evaluated daily. The values were expressed as mean ± SD of three independent experiments. Statistical analysis was performed using ANOVA followed by the Tukey’s test for multiple comparison at p < 0.05. a, b, c, and d indicate statistically significant differences on eGFP expression level of fcpA, CIP1, GLNA-501, and GLNA-997-driven constructs at Day 6 and Day 11

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