Table 2 Similarity (% of identity) of ORFs in the conjugative gene cluster between pLU4 and other lactic acid bacterial plasmids

ORF3 (61)

100% (P3)

100% (P22)

–

57%

–

ATPase (226)

95% (ATPase)

86% (ATPase)

–

62% (ATPase)

–

ORF5 (92)

95% (P5)

97% (P24)

93% (P40)

60% (P11)

NH (64, P3)

ORF6 (75)

94% (69, P6)

94% (69, P25)

94% (69, P39)

53% (94, P10)

57% (94, P4)

TrsA (687)

97% (691, Nickase)

96% (TraA)

67% (686, TraA)

pseudogene

50% (680, TraA)

ORF8 (103)

–

100% (P27)

71% (112, P37)

59% (106, P7)

52% (114, P6)

ORF9 (204)

98% (P8)

95% (P28)

94% (P36)

62% (208, P6)

50% (201, P7)

TrsB (111)

99% (TrsB)

100% (TraB)

100% (TraB)

76% (114, TraB)

72% (114, TraB)

TrsC (120)

100% (TrsC)

100% (TraC)

98% (TraC)

80% (116, TraC)

72% (115, TraC)

TrsD (219)

99% (TrsD)

99% (TraD)

98% (TraD)

82% (217, TraD)

79% (217, TraD)

TrsE (672)

98% (TrsE)

97% (TraE)

97% (TraE)

86% (TraE)

84% (TraE)

TrsF (472)

97% (TrsF)

91% (TraF)

92% (TraF)

59% (TraF)

51% (TraF)

PGH (385)

98% (P14)

97% (PGH)

96% (384, PGH)

77% (384, PGH)

54% (PGH)

ORF16 (205)

93% (P15)

95% (P35)

92% (P29)

54% (P38)

48% (P14)

TRX (122)

89% (110, TRX)

90% (TRX)

91% (TRX)

60% (94, TRX)

43% (TRX)

TrsJ (156)

96% (TrsJ)

88% (TraJ)

88% (TraJ)

52% (152, TraJ)

50% (152, TraJ)

ORF19 (78)

–

–

–

–

–

TrsK (516)

97% (514, TrsK)

98% (518, TraK)

93% (503, TraK)

82% (514, TraK)

77% (530, TraK)

ORF21 (129)

98% (P19)

98% (135, P39)

43% (124, P25)

53% (131, P34)

45% (131, P18)

TrsL (279)

99% (TrsL)

99% (TraL)

96% (285, TraL)

43% (278, TraL)

44% (278, TraL)

ORF23 (136)

92% (P21)

92% (134, P41)

78% (P23)

–

–

TrsI (711)

96% (TrsI)

97% (712, TraI)

96% (TraI)

70% (722, TraI)

60% (725, TraI)

ORF25 (71)

55% (P23)

55% (P43)

55% (P21)

60% (72, P31)

58% (69, P21)

LtrC (374)

83% (LtrC)

87% (LtrC)

–

77% (469, LtrC)

58% (355, LtrC)