Fig. 3

Amelioration of the intestinal inflammatory lesion and obstruction of the infiltration of mast cells and eosinophils by Rosae extract. The small intestines (jejunum) were taken from the respective group of mice one day after the fifth cOVA challenge and fixed with formalin and sectioned with microtome. The thin tissue sections were stained with hematoxylin plus eosin (H&E) and observed under the microscope for inflammatory lesions (A, left) and for eosinophils (stained dark red) (A, center). They were also stained with toluidine solution and observed for mast cells (stained deep blue) (A right). The numbers of mast cells (B, left) and eosinophils (B, right) in high power fields were counted, and the average numbers were plotted. Cell numbers in at least five different HPFs were counted, and the means and SDs were calculated. Group description is shown as shown in Fig. 1A. Asterisk (*) denotes p < 0.05 by Student’s t test