Fig. 6

Inhibition of activation/proliferation of DO11.10 T cells induced by cOVA in vivo. DO11.10 TCR Tg T cells labeled with CFSE were adoptively transferred and systemically stimulated with cOVA plus alum with or without treatment with either Rosae extract or Dexa as depicted in (A). The cells prepared from the whole-body LNs were stained with anti-CD4 plus KJ1-26 mAbs and analyzed with flow cytometry. Dot plots show the population of KJ1-26⁺ (DO11.10) T cells among the gated total CD4⁺ T cells and the extent of CFSE dilution in those cells (B). The cells from the LNs were cultured ex vivo with the DO11.10-specific peptide for 3 days, and the levels of IL-4, IL-5 and IFN-γ were measured with ELISA (C). The experiments were carried out with three mice per group