Comparison of NheA toxin production and doubling time between Bacillus cereus and Bacillus thuringiensis

Applied Biological Chemistry

Table 2 Oligonucleotide sequences of primers used in this study

Gene primer		Sequences (5′ → 3′)	Amplicon size (bp)	References
nheA	NA-F1	ATT ACA GGG TTA TTG GTT ACA GCA GT	475	[49]
	NA-R1	AAT CTT GCT CCA TAC TCT CTT GGA TGC T
nheB	NB-F1	GTG CAG CAG CTG TAG GCG GT	328
	NB-R1	ATG TTT TTC CAG CTA TCT TTC GCA AT
nheC	NC-F1	GCG GAT ATT GTA AAG AAT CAA AAT GAG GT	557
	NC-R1	TTT CCA GCT ATC TTT CGC TGT ATG TAA AT
hblA	FhblA	GCA AAA TCT ATG AAT GCC TA	884	[11]
	RhblA	GCA TCT GTT CGT AAT GTT TT
hblC	FhblC	CCT ATC AAT ACT CTC GCA A	695
	RhblC	TTT CCT TTG TTA TAC GCT GC
hblD	HDF	ACC GGT AAC ACT ATT CAT GC	829
	HDR	GAG TCC ATA TGC TTA GAT GC
cytK	FCytK	CGA CGT CAC AAG TTG TAA CA	565	[11]
	R2CytK	CGT GTG TAA ATA CCC CAG TT
groEL	Rba1F	TGC AAC TCT ATT ACG ACA AGC T	238	[40]
	Rba1R	TTA CCA ACG CGC TCC ATT GCT T		[50]
ces	RCesF1	GGT GAC ACA TTA TCA TAT AAG GTG	120	[50]
	RCesR2	ATT CAA CAT AAT ATT ATA CGC CGT
cry1	c1Aa	ATT CGC TAG GAA CCA AGC	398	[41]
	c1Ad	AAT CCG GTC CCC ATA CAC