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Applied Biological Chemistry

Table 5 Growth and NheA toxin production by B. cereus and B. thuringiensis strains

From: Comparison of NheA toxin production and doubling time between Bacillus cereus and Bacillus thuringiensis

Strain

Toxin production (ABS)a

Cell number (×108 CFU)

NheA-specific production (ABS/108 CFU)

Doubling time (min)

Bacillus cereus ATCC 14579

1.20

6.33

0.19

18

Bacillus cereus ATCC 11778

1.04

3.38

0.31

19

Bacillus thuringiensis KCTC 1510

0.67

2.43

0.28

22

Bacillus thuringiensis KCTC 1513

0.84

5.88

0.14

25

Bacillus thuringiensis pesticide isolate 1 (var. kurstaki)

2.24

2.35

0.95

20

Bacillus thuringiensis pesticide isolate 2 (var. kurstaki)

1.87

2.85

0.66

22

Bacillus thuringiensis pesticide isolate 3 (var. aizawai)

0.91

2.50

0.36

25

Bacillus thuringiensis pesticide isolate 4 (var. aizawai)

1.21

2.66

0.45

26

  1. Cells were grown in Brain–Heart-Infusion broth and incubated at 37 °C with moderate shaking. Results of NheA toxin production, viable cell count, and doubling time are means of duplicates
  2. Absorbance results >0.2 are considered to be positive based on ELISA. O/S, off scale, absorbance >3.0
  3. aThe amount of NheA toxin production was detected using the Tecra BDE-VIA kit and was estimated from absorbance at 414 nm
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