From: Isolation and purification of phycocyanin from cyanobacteria of a mangrove forest
Purification step | Purity ratio A620/A280 | Separation factor A620/A652 | C-PC (mg ml−1) | Recovery of C-PC (%) |
---|---|---|---|---|
(1) Crude extract | 0.8 | 2.4 | 0.2 | 100 |
(2) Ammonium sulphate precipitation with 25% saturation | 0.9 | 2.4 | 0.3 | 93 |
(3) Ammonium sulphate precipitation with 50% saturation | 2.4 | 2.9 | 0.3 | 83 |
(4) DEAE-cellulose-52 | 4.3 | 4.6 | 0.4 | 48 |