Determination by molecular methods of genetic and epigenetic changes caused by heavy metals released from thermal power plants

Applied Biological Chemistry

Table 1 Primers used in RT-PCR

Primer name	Sequence 5′ → 3′	TM (°C)	GC%
ZmGF14-4F	GAACCTCTTATCTGTTGCCT	50	45
ZmGF14-4R	GATGACTAGATGCCAGTTCC	52	50
ZmGF14-6F	GCATGCAGAAGGGTTGAGCA	56	57
ZmGF14-6R	TCAGGGCTCATCTAGCTGGTCCTG	61	58
Ta14S-F1	ACGACTCAAGCGAGGGGCA	55	63
Ta14S-R1	CGCCTGCTACGCTACAAGGAC	58	62
Ta14S-F2	GTCAATGACCGTTGCAATGTG	52	48
Ta14S-R2	GCCACCACCACCACTGTATG	56	60
β-actin-F	TTTGAAGAGTCGGTGAAGGG	52	50
β-actin-R	TTTCATACAGCAGGCAAGCA	50	45