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Applied Biological Chemistry

Table 1 Analysis of variance of the regression parameters for the predicted response surface quadratic models of the conditions of enzymatic hydrolysis and β-glucan contents of the Ganoderma lucidum extracts obtained under optimal conditions

From: Improvement in β-glucan extraction from Ganoderma lucidum with high-pressure steaming and enzymatic pre-treatment

Response

Polynomial equations

R 2

Significance

Optimum condition

Predicted value (A)

Empirical value (B)

B/A × 100

X 1

X 2

X 3

β-Glucan (g/100 g)

Y1 = 1.52125 + 0.129875X1 + 8.9875X2 + 0.893125X3 − 0.015X1X2 − 0.00765X1X3 − 0.185X2X3 − 0.0032X 21  − 4.78X 22  − 0.07625X 23

0.9561

0.0020

15.51

0.84

4.16

8.22

8.05

97.93

  1. The predicted value was calculated using the predicted equation for the response variables
  2. X1, high-pressure steaming time (min); X2, enzyme concentration (g/100 g); X3, enzymatic hydrolysis time (h)
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