Table 3 Alteration in α helix and β sheet content of native and UV-C irradiated α crystallin from 0 min to 90 min, obtained from FTIR spectroscopy in the Amide I region (1600–1700 cm−1)
From: Aberration in the structural paradigm of lens protein α crystallin by UV-C irradiation
Sample | Amide I region (1600–1700 cm−1) | ||
|---|---|---|---|
Protein | α-Helix (%) | FWHM | β-sheet (%) |
Native | 14.51 | 193.52 | 36.75 |
30 min | 17.27 | 95.06 | 32.60 |
60 min | 15.52 | 90.72 | 31.11 |
90 min | 4.71 | 83.8 | 10.10 |