Table 1 Plasmids, Escherichia coli strains, and primers used in this study
From: Microbial synthesis of hydroxytyrosol and hydroxysalidroside
Plasmids or E. coli strain | Relevant properties or genetic marker | Source or reference |
|---|---|---|
Plasmids | ||
pACYCDuet | P15A ori, Cmr | Novagen |
pCDFDuet | CloDE13 ori, Strr | Novagen |
pA-aroG-tyrA | pACYCDuet carrying aroG and tyrA | [17] |
pA-aroGfbr-tyrAfbr | pACYCDuet carrying feedback inhibition free of aroG and tyrA | [17] |
pA-aroGfbr-ppsA-tktA-tyrAfbr | pACYCDuet carrying ppsA, tktA, and feedback inhibition free of aroG and tyrA | [17] |
pC-TDC-TYO | pCDFDuet carrying tyrosine decarboxylase (TDC) from Papaver somniferum and tyrosine oxidase (TYO) from Micrococcus luteus | This study |
pE-HpaBC | pET-duet carrying HpaBC from Escherichia coli | This study |
Strains | ||
BL21 (DE3) | F− ompT hsdS B (r −B m −B ) gal dcm lon (DE3) | |
B-TP | BL21(DE3) ΔtyrR::FRT-ΔPheA::FRT-kanR-FRT[17] | [17] |
B-TPF | BL21(DE3) ΔtyrR::FRT-ΔPheA::FRT-ΔfeaB::FRT-kanR-FRT | [21] |
B-TYS1 | BL21 (DE3) harboring pC-TDC-TYO | This study |
B-TYS2 | BL21 (DE3) harboring pC-TDC-TYO, and pE-HpaBC | This study |
B-TYS3 | BL21 (DE3) harboring pA-aroG-tyrA, pC-TDC-TYO, and pE-HpaBC | This study |
B-TYS4 | BL21 (DE3) harboring pA-aroGfbr-tyrAfbr, pC-TDC-TYO, and pE-HpaBC | This study |
B-TYS5 | B-TP harboring pA-aroGfbr-tyrAfbr, pC-TDC-TYO, and pE-HpaBC | This study |
B-TYS6 | B-TPF harboring pA-aroGfbr-tyrAfbr, pC-TDC-TYO, and pE-HpaBC | This study |