Comparison of the retention rates of thiamin, riboflavin, and niacin between normal and high-oleic peanuts after roasting

Applied Biological Chemistry

Table 1 HPLC operating condition for vitamin thiamin, riboflavin, and niacin analyses

	Thiamin and Niacin	Riboflavin
Instrument	Agilent 1100 series	Agilent 1100 series
Column	YMC-Pack ODS AM (250 mm × 4.6 mm, 5 μm)	YMC PRO RS C18 (250 mm × 4.6 mm, 5 μm)
Column temp.	40 °C	40 °C
Detector	Diode array detector (270 nm)	FLD (Ex = 445 nm, Em = 530 nm)
Flow rate	0.8 mL/min	0.8 mL/min
Mobile phase	A: 5 mM sodium 1-hexanesulfonate (acetic acid 7.5 mL + triethylamine 0.2 mL/1 L)	10 mM NaH₂PO₄ (pH 5.5): MeOH = 75:25 (v/v)
Mobile phase	B: 100% MeOH
Gradient profile	0 min: A 100%	Isocratic
	8 min: A 100%
	20 min: A 75% + B 25%
	30 min: A 55% + B 45%
	31 min: A 100%
	45 min: A 100%
Injection volume	20 μL	20 μL