Markers for distinguishing Orostachys species by SYBR Green-based real-time PCR and verification of their application in commercial O. japonica food products

Applied Biological Chemistry

Table 4 Slope, correlation coefficient, efficiency, and Ct values obtained by qPCR assay using the developed primers

Target species	Primer	Y (slope)	R² (correlation coefficients)	Efficiency (%)	Ct^a
O. japonica	trnT-L	− 3.437	0.998	95.23	21.4
	ndhF	− 3.663	0.999	87.5	23.1
	rpoB	− 3.348	0.999	98.9	22.8
O. iwarenge and O. malacophyllus	matK	− 3.652/ − 3.642	0.998/ 0.999	84.26/ 88.88	24
	trnS-G	− 3.489/ − 3.525	0.999/ 0.999	93.46/ 92.18	24.6
	trnQ-rps16	− 3.562/ − 3.649	0.999/ 0.999	90.87/ 87.97	23.3
	ycf2	− 3.395/ − 3.497	0.999/ 0.999	97.04/ 93.17	21.6
O. latiellipticus and O. ramosus	matK	− 3.61/ − 3.648	0.999/ 0.999	89.2/ 87.98	23.2
	trnT-L	− 3.385/ − 3.286	0.999/ 0.999	97.41/ 101.54	24
	trnQ-rps16	− 3.766/ − 3.448	0.996/ 0.997	84.31/ 95.01	26.7
	ITS	− 3.307/ − 3.129	0.998/ 0.998	100.64/ 108.73	23.2
O. margaritifolius	ndhF	− 3.899	0.998	80.49	24.4

^a Ct values represent the limit of detection of 10 pg of DNA from each species using the species-specific primers