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Applied Biological Chemistry
Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Effect of DNA extraction methods on the detection of porcine ingredients in halal cosmetics using real-time PCR

Fig. 1

(A) Standard curve generated by real-time PCR using known amounts of template DNA and (B) agarose gel electrophoresis of real-time PCR products. Real-time PCR was performed with serially diluted (tenfold) template DNA ranging from 2.28 × 1010 copies/tube to 2.28 × 1010 copies/tube. Negative template control consisted of the reaction mixture without template DNA. Lane 1: 100-bp ladder. Lanes 2, 3, and 4: samples containing template DNA (2.28 × 102, 2.28 × 101, and 2.28 × 100 copies/tube, respectively). Lane 5: negative template control

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